腺嘌呤磷酸核糖转移酶(APRT)活性蛋白

Active Adenine Phosphoribosyltransferase (APRT)

AMP; APRTase

  • 腺嘌呤磷酸核糖转移酶(APRT)活性蛋白 产品包装(模拟)
  • 腺嘌呤磷酸核糖转移酶(APRT)活性蛋白 产品包装(模拟)
  • 腺嘌呤磷酸核糖转移酶(APRT)活性蛋白 Gene sequencing
  • APC310Hu01.jpg SDS-PAGE图
  • 腺嘌呤磷酸核糖转移酶(APRT)活性蛋白 Figure. Western Blot; Sample: Recombinant APRT, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Adenine Phosphoribosyltransferase (APRT) is an enzyme involved in the purine nucleotide salvage pathway. It functions as a catalyst in the reaction between adenine and phosphoribosyl pyrophosphate (PRPP) to form AMP. Besides, Vascular Cell Adhesion Molecule 1 (VCAM1) has been identified as an interactor of APRT, thus a binding ELISA assay was conducted to detect the interaction of recombinant human APRT and recombinant human VCAM1. Briefly, APRT were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to VCAM1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-APRT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of APRT and VCAM1 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

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编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
APC310Hu01 腺嘌呤磷酸核糖转移酶(APRT)活性蛋白 Cell culture; Activity Assays.
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