醛脱氢酶1家族成员A1(ALDH1A1)活性蛋白

Active Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1)

ALDH11; ALDH1; ALDC; PUMB1; RALDH1; Retinaldehyde Dehydrogenase 1; Aldehyde dehydrogenase, cytosolic; Retinal dehydrogenase 1

  • 醛脱氢酶1家族成员A1(ALDH1A1)活性蛋白 产品包装(模拟)
  • 醛脱氢酶1家族成员A1(ALDH1A1)活性蛋白 产品包装(模拟)
  • APE824Hu01.jpg Figure. SDS-PAGE
  • 醛脱氢酶1家族成员A1(ALDH1A1)活性蛋白 WB图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

ALDH1A1 (Retinal dehydrogenase 1) which belongs to the aldehyde dehydrogenase family, is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. ALDH1A1 converts retinaldehyde to retinoic acid. IL21 (interleukin 21) was identified as an interactor of ALDH1A1 through Affinity Capture-MS. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human ALDH1A1 and recombinant human IL21. Briefly, ALDH1A1 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL ALDH1A1 were then transferred to IL21-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ALDH1A1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of ALDH1A1 and IL21 was shown in Figure 1, and this effect was in a dose dependent manner.

The activity of recombinant human ALDH1A1 was also measured by its ability to produce NADH during the oxidation of propionaldehyde. The reaction was performed in 0.1 M Tris, 0.1 M KCl, 2mM DTT, pH 8.5 (assay buffer), initiated by addition 50 μL of various concentrations of ALDH1A1 (diluted by assay buffer) to 50 µL of substrate mixture 2 mM beta-NAD and 20 mM propionaldehyde. The final well serves as a negative control with no ALDH1A1, replaced with 50 μl assay buffer. Read at a wavelength of 340 nm in kinetic mode for 5 minutes. The specific activity of recombinant human ALDH1A1 is > 130 pmol/min/µg.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPE824Hu01 醛脱氢酶1家族成员A1(ALDH1A1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APE824Hu01 醛脱氢酶1家族成员A1(ALDH1A1)活性蛋白 Cell culture; Activity Assays.
RPE824Hu02 醛脱氢酶1家族成员A1(ALDH1A1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAE824Hu01 醛脱氢酶1家族成员A1(ALDH1A1)多克隆抗体 WB; IHC
PAE824Hu02 醛脱氢酶1家族成员A1(ALDH1A1)多克隆抗体 WB; IHC; ICC; IP.
MAE824Hu21 醛脱氢酶1家族成员A1(ALDH1A1)单克隆抗体 WB; IHC; ICC; IP.
SEE824Hu 醛脱氢酶1家族成员A1(ALDH1A1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LME824Hu 醛脱氢酶1家族成员A1(ALDH1A1)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
Oncology Letters Upregulation of stem cell markers ALDH1A1 and OCT4 as potential biomarkers for the early detection of cervical carcinoma [Doi: 10.3892/ol.2018.9381]
Journal of Oncology High Aldehyde Dehydrogenase Levels Are Detectable in the Serum of Patients with Lung Cancer and May Be Exploited as Screening Biomarkers []
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