芳基硫酸酯酶F(ARSF)活性蛋白

Active Arylsulfatase F (ARSF)

ASF

  • 芳基硫酸酯酶F(ARSF)活性蛋白 产品包装(模拟)
  • 芳基硫酸酯酶F(ARSF)活性蛋白 产品包装(模拟)
  • APG613Hu01.jpg SDS-PAGE图
  • 芳基硫酸酯酶F(ARSF)活性蛋白 Figure. Western Blot; Sample: Recombinant ARSF, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

ARSF (Arylsulfatase F) is a member of the sulfatase gene family, and more specifically, the arylsulfatase subfamily. Sulfatases are essential for the correct composition of bone and cartilage matrix. The activity of this protein, unlike that of arylsulfatase E, is not inhibited by warfarin. Besides, EP300 (E1A binding protein p300) has been identified as an interactor of ARSF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ARSF and recombinant human EP300. Briefly, ARSF were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to EP300-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-ARSF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of ARSF and EP300 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

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编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPG613Hu01 芳基硫酸酯酶F(ARSF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
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