碳酸酐酶Ⅱ(CA2)活性蛋白

Active Carbonic Anhydrase II (CA2)

CA-II; CAII; Car2; CAC; Carbonate dehydratase II; Carbonic anhydrase C

  • 碳酸酐酶Ⅱ(CA2)活性蛋白 产品包装(模拟)
  • 碳酸酐酶Ⅱ(CA2)活性蛋白 产品包装(模拟)
  • APA782Ra01.jpg SDS-PAGE图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

CA2 (Carbonic anhydrase 2) is an enzyme that catalyzes reversible hydration of carbon dioxide. It is essential for bone resorption and osteoclast differentiation and contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. It is widely accepted that CA2 also catalyzes hydrolysis of p-Nitrophenyl Acetate. Thus, a hydration assay was conducted to test the catalytic activity of CA2 using 4-Nitrophenyl Acetate (4-NPA) as substrate. Briefly, different concentrations of CA2 were incubated with 1mM 4-NPA in reaction buffer. The absorbance at the wavelength of 400nm was read per hour, and the result was shown in figure 1. It is obvious that CA2 catalyzes hydrolysis of p-Nitrophenyl Acetate.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
NPA782Ra01 碳酸酐酶Ⅱ(CA2)天然蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA782Ra01 碳酸酐酶Ⅱ(CA2)活性蛋白 Cell culture; Activity Assays.
RPA782Ra01 碳酸酐酶Ⅱ(CA2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA782Ra02 碳酸酐酶Ⅱ(CA2)多克隆抗体 WB; IHC; ICC; IP.
PAA782Ra01 碳酸酐酶Ⅱ(CA2)多克隆抗体 WB; IHC; ICC; IP.
LAA782Ra71 碳酸酐酶Ⅱ(CA2)多克隆抗体(生物素标记) WB; IHC; ICC.
SEA782Ra 碳酸酐酶Ⅱ(CA2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA782Ra 碳酸酐酶Ⅱ(CA2)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA782Ra01 碳酸酐酶Ⅱ(CA2)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Proteomics Clin Appl.  Identification of potential breast cancer markers in nipple discharge by protein profile analysis using two-dimensional nano-liquid chromatography/nanoelectrospray ionization-mass spectrometry. [Pubmed:26970563]
PROTEOMICS - Clinical Applications Comparative mass spectrometric and immunoassay-based proteome analysis in serum ofDuchenne muscular dystrophy patients. [pubmed:26680509]
Cell & Molecular Biology Identification of potential breast cancer markers in nipple discharge by protein profile analysis using two‐dimensional nano‐liquid chromatography/nanoelectrospray … [doi:10.1002]
留言咨询