Active Colony Stimulating Factor 1, Macrophage (MCSF)
CSF1; M-CSF; Lanimostim; Processed macrophage colony-stimulating factor 1
- 物种Mus musculus (Mouse，小鼠)相同的名称，不同的物种。
- 品牌商CLOUD-CLONE CORP.(CCC,美国)
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 纯度> 95%
- 应用Cell culture; Activity Assays.
- 下载英文说明书 中文说明书
- Gene sequencing
- Figure. Western Blot; Sample: Recombinant MCSF, Mouse.
- 通过ISO 9001、ISO 13485质量体系认证
Macrophage Colony Stimulating Factor (M-CSF), also known as CSF-1,is a secreted cytokine which influences hematopoietic stem cells to differentiate into macrophages or other related cell types. M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It can also affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor (CSF1R or M-CSF-R encoded by the c-fms proto-oncogene), M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy. Besides, Colony Stimulating Factor Receptor, Macrophage (M-CFS-R) has been identified as an interactor of M-CSF, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse M-CSF and recombinant mouse MCFSR Briefly, M-CSF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to M-CFS-R-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-M-CSF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of M-CSF and M-CFS-R was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
|编号||适用物种：Mus musculus (Mouse，小鼠)||应用|
|RPA090Mu01||巨噬细胞集落刺激因子(MCSF)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA090Mu02||巨噬细胞集落刺激因子(MCSF)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|APA090Mu01||巨噬细胞集落刺激因子(MCSF)活性蛋白||Cell culture; Activity Assays.|
|PAA090Mu01||巨噬细胞集落刺激因子(MCSF)多克隆抗体||WB; IHC; ICC; IP.|
|PAA090Mu02||巨噬细胞集落刺激因子(MCSF)多克隆抗体||WB; IHC; ICC; IP.|
|SEA090Mu||巨噬细胞集落刺激因子(MCSF)检测试剂盒(酶联免疫吸附试验法)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|MEA090Mu||巨噬细胞集落刺激因子(MCSF)检测试剂盒(酶联免疫吸附试验法,小样本)||Enzyme-linked immunosorbent assay for Antigen Detection.|