细胞色素P450家族成员1A1(CYP1A1)活性蛋白

Active Cytochrome P450 1A1 (CYP1A1)

CYP1; AHH; AHRR; CP11; CYP1; P1-450; P450-C; P450DX; Cytochrome P450,Subfamily I(Aromatic Compound-Inducible),Polypeptide 1

  • 细胞色素P450家族成员1A1(CYP1A1)活性蛋白 产品包装(模拟)
  • 细胞色素P450家族成员1A1(CYP1A1)活性蛋白 产品包装(模拟)
  • APD295Ra01.jpg Figure. SDS-PAGE
  • 细胞色素P450家族成员1A1(CYP1A1)活性蛋白 Figure. Western Blot; Sample: Recombinant CYP1A1, Rat.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Cytochrome P450 1A1 (CYP1A1) is a member of Cytochromes P450 superfamily of enzymes. Cytochromes P450 are a group of heme-thiolate monooxygenases. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. CYP1A1 is also known as AHH (aryl hydrocarbon hydroxylase). It is involved in the metabolic activation of aromatic hydrocarbons (polycyclic aromatic hydrocarbons, PAH). Besides, Heat Shock 70kDa Protein 4 (HSPA4) has been identified as an interactor of CYP1A1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CYP1A1 and recombinant rat HSPA4. Briefly, CYP1A1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HSPA4-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CYP1A1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of CYP1A1 and HSPA4 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
RPD295Ra01 细胞色素P450家族成员1A1(CYP1A1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APD295Ra01 细胞色素P450家族成员1A1(CYP1A1)活性蛋白 Cell culture; Activity Assays.
PAD295Ra01 细胞色素P450家族成员1A1(CYP1A1)多克隆抗体 WB; IHC; ICC; IP.
MAD295Ra21 细胞色素P450家族成员1A1(CYP1A1)单克隆抗体 WB; IHC; ICC; IP.
SED295Ra 细胞色素P450家族成员1A1(CYP1A1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD295Ra 细胞色素P450家族成员1A1(CYP1A1)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSD295Ra01 细胞色素P450家族成员1A1(CYP1A1)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Journal of Nutrition Apiaceous Vegetable Consumption Decreases PhIP-Induced DNA Adducts and Increases Methylated PhIP Metabolites in the Urine Metabolome in Rats [Pubmed:25733458]
The Journal of Nutrition Apiaceous Vegetable Consumption Decreases PhIP-Induced DNA Adducts and Increases Methylated PhIP Metabolites in the Urine Metabolome in Rats1,2,3 [PubMed: 25733458]
Molecular Nutrition Food Research Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats [Pubmed:27133590]
International Journal of Chemical and Natural Science Caspase-3, Bcl-2, p53, CYP1A1 and COX -2 as a potential target in chemoprevention of Benzo (a) pyrene-induced lung carcinogenesis in mice: Role of thymoquinone [portal:uploads]
International Journal of Pharma Sciences Chemopreventive Role of Curcumin in benzo(A)pyrene induced Lung Carcinogenesis in mice via-modulation of Bcl-2, p53, Caspase-3, Cyp1A1, COX-2 and antioxidant defense system in Lung tissues [portal:uploads]
Molecular Nutrition & Food Research Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats. [pubmed:27133590]
Biochemistry (Mosc) Cytochrome P450 1A1 (CYP1A1) Catalyzes Lipid Peroxidation of Oleic Acid-Induced HepG2 Cells [Pubmed:29738693]
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