细胞色素P450家族成员1A2(CYP1A2)活性蛋白

Active Cytochrome P450 1A2 (CYP1A2)

CP12; P3-450; P450(PA); Cytochrome P450,Family 1,Subfamily A,Polypeptide 2

  • 细胞色素P450家族成员1A2(CYP1A2)活性蛋白 产品包装(模拟)
  • 细胞色素P450家族成员1A2(CYP1A2)活性蛋白 产品包装(模拟)
  • 细胞色素P450家族成员1A2(CYP1A2)活性蛋白 Gene sequencing
  • APD294Hu01.jpg Figure. SDS-PAGE
  • 细胞色素P450家族成员1A2(CYP1A2)活性蛋白 Figure. Western Blot; Sample: Recombinant CYP1A2, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

CYP1A2 (Cytochrome P450 1A2) belongs to the group of proteins which contains heme as a cofactor. CYP1A2 oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Besides, ASAH1 (Acid ceramidase) has been identified as an interactor of CYP1A2 through affinity capture-MS. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human CYP1A2 and recombinant human ASAH1. Briefly, CYP1A2 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ASAH1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CYP1A2 mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of CYP1A2 and ASAH1 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
RPD294Hu01 细胞色素P450家族成员1A2(CYP1A2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APD294Hu01 细胞色素P450家族成员1A2(CYP1A2)活性蛋白 Cell culture; Activity Assays.
RPD294Hu02 细胞色素P450家族成员1A2(CYP1A2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
CPD294Hu21 细胞色素P450家族成员1A2(CYP1A2)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAD294Hu01 细胞色素P450家族成员1A2(CYP1A2)多克隆抗体 WB; IHC; ICC; IP.
PAD294Hu02 细胞色素P450家族成员1A2(CYP1A2)多克隆抗体 WB; IHC; ICC; IP.
PAD294Hu08 细胞色素P450家族成员1A2(CYP1A2)多克隆抗体 WB; IHC; ICC; IP.
LAD294Hu71 细胞色素P450家族成员1A2(CYP1A2)多克隆抗体(生物素标记) WB; IHC; ICC.
SED294Hu 细胞色素P450家族成员1A2(CYP1A2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD294Hu 细胞色素P450家族成员1A2(CYP1A2)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Environmental Monitoring and Assessment Differential expression of HO-1 and CYP1A2 during up-regulation of ERK in stressed fish hepatocytes [Pubmed:25471622]
The Journal of Nutrition Apiaceous Vegetable Consumption Decreases PhIP-Induced DNA Adducts and Increases Methylated PhIP Metabolites in the Urine Metabolome in Rats1,2,4 [PubMed: 25733458]
Molecular Nutrition Food Research Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats [Pubmed:27133590]
Molecular Nutrition & Food Research Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats. [pubmed:27133590]
 Journal of FisheriesSciences. com Upregulation of HSP70 Extends Cytoprotection to Fish Brain under Xenobiotic Stress [b0407ebde042c4f61b728bc11a586e6c]
Toxicology Liver lobe and strain differences in the activity of murine cytochrome p450 enzymes [Pubmed:29879457]
Stammesspezifische Unterschiede in der analgetischen Wirkung von Buprenorphin bei der Maus []
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