二酰甘油激酶ε(DGKe)活性蛋白

Active Diacylglycerol Kinase Epsilon (DGKe)

DAGK5; DGK; Diglyceride kinase epsilon

  • 二酰甘油激酶ε(DGKe)活性蛋白 产品包装(模拟)
  • 二酰甘油激酶ε(DGKe)活性蛋白 产品包装(模拟)
  • APE848Hu01.jpg SDS-PAGE图
  • 二酰甘油激酶ε(DGKe)活性蛋白 Figure. Western Blot; Sample: Recombinant DGKe, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

DGKe (Diacylglycerol kinase epsilon) is an enzyme that mainly involved in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. PDHA1 (Pyruvate dehydrogenase E1 component subunit alpha) has been proven to be an interactor of DGKe through Affinity Capture-MS. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human DGKe and recombinant human PDHA. Briefly, DGKe were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL DGKe were then transferred to PDHA-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-DGKe pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of DGKe and PDHA was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
RPE848Hu01 二酰甘油激酶ε(DGKe)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APE848Hu01 二酰甘油激酶ε(DGKe)活性蛋白 Cell culture; Activity Assays.
PAE848Hu01 二酰甘油激酶ε(DGKe)多克隆抗体 WB; IHC; ICC; IP.
留言咨询