谷胱甘肽S转移酶μ1(GSTM1)活性蛋白
Active Glutathione S Transferase Mu 1 (GSTM1)
GST-M1; GST1; GSTM1-1; GSTM1a-1a; GSTM1b-1b; GTH4; GTM1; H-B; MU1; GST HB subunit 4
- 编号APA658Ra01
- 物种Rattus norvegicus (Rat，大鼠) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 95%
- 等电点8.3
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 2256 ¥ 5640 ¥ 11280 ¥ 33840 ¥ 84600
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产品包装(模拟)
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产品包装(模拟)
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SDS-PAGE图
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Figure. Western Blot; Sample: Recombinant GSTm1, Rat.
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Glutathione S-transferase Mu 1 (GSTM1) belongs to the mu class. GSTM1 can detoxifying carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Besides, uncoupling Protein 1 (UCP1) has been identified as an interactor of GSTM1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat GSTM1 and recombinant rat UCP1. Briefly, GSTM1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to UCP1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-GSTM1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of GSTM1 and UCP1 was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA658Ra01 | 谷胱甘肽S转移酶μ1(GSTM1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA658Ra01 | 谷胱甘肽S转移酶μ1(GSTM1)活性蛋白 | Cell culture; Activity Assays. |
| PAA658Ra01 | 谷胱甘肽S转移酶μ1(GSTM1)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA658Ra71 | 谷胱甘肽S转移酶μ1(GSTM1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEA658Ra | 谷胱甘肽S转移酶μ1(GSTM1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA658Ra | 谷胱甘肽S转移酶μ1(GSTM1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA658Ra01 | 谷胱甘肽S转移酶μ1(GSTM1)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
| 杂志 | 参考文献 |
| Mol Neurobiol | Human Glutathione S-Transferase Enzyme Gene Polymorphisms and Their Association With Neurocysticercosis [Pubmed:27021019] |
| CHEMICAL SENSES | Interactions Between Odorants and Glutathione Transferases in the Human Olfactory Cleft [Pubmed: 32822470] |
