白介素6受体(IL6R)活性蛋白

Active Interleukin 6 Receptor (IL6R)

CD126; gp80; IL6R; IL-6R-1; IL-6R-Alpha; IL6RA; IL-6 receptor subunit alpha; Membrane glycoprotein 80

  • 白介素6受体(IL6R)活性蛋白 产品包装(模拟)
  • 白介素6受体(IL6R)活性蛋白 产品包装(模拟)
  • APB815Mu02.jpg Figure. SDS-PAGE; Sample: Active recombinant IL6R, Mouse.
  • 白介素6受体(IL6R)活性蛋白 Figure. Western Blot; Sample: Recombinant IL6R, Mouse.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

IL6R (Interleukin-6 receptor subunit alpha) is part of the receptor for interleukin 6. The interaction of IL6R and IL6 may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Thus, a binding ELISA assay was conducted to detect the association of IL6R with IL6. Briefly, IL6R were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL6-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL6R pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL6R with IL6 was shown in Figure 1 and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPB815Mu02 白介素6受体(IL6R)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB815Mu02 白介素6受体(IL6R)活性蛋白 Cell culture; Activity Assays.
RPB815Mu01 白介素6受体(IL6R)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB815Mu02 白介素6受体(IL6R)多克隆抗体 WB; IHC; ICC; IP.
PAB815Mu01 白介素6受体(IL6R)多克隆抗体 WB; IHC; ICC; IP.
LAB815Mu71 白介素6受体(IL6R)多克隆抗体(生物素标记) WB; IHC; ICC.
SEB815Mu 白介素6受体(IL6R)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB815Mu 白介素6受体(IL6R)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
J Orthop Res Interleukin‐6 and interleukin‐6 receptor expression, localization, and involvement in pain‐sensing neuron activation in a mouse intervertebral disc injury model [PubMed: 26010074]
Gene.  The IL6R gene polymorphisms are associated with sIL-6R, IgE and lung function in Chinese patients with asthma [Pubmed:26997259]
Cytokine Interleukin-6 participates in human pancreatic stellate cell activation and collagen I production via TGF-¦Â1/Smad pathway [33893003]
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