神经氨酸酶(NEU)活性蛋白
Active Neuraminidase (NEU)
NEU1; SIAL1; Sialidase 1; Lysosomal Sialidase; Acetylneuraminyl hydrolase; G9 sialidase; N-acetyl-alpha-neuraminidase 1
- 编号APB611Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 90%
- 等电点5.5
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1920 ¥ 4800 ¥ 9600 ¥ 28800 ¥ 72000
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产品包装(模拟)
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产品包装(模拟)
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SDS-PAGE图
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Figure. Western Blot; Sample: Recombinant NEU, Human.
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通过ISO 9001、ISO 13485质量体系认证
活性实验
NEU (Sialidase-1) is an enzyme that catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (CTSA). Thus a binding ELISA assay was conducted to detect the interaction of recombinant human NEU and recombinant human CTSA. Briefly, NEU were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CTSA-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-NEU pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of NEU and CTSA was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB611Hu05 | 神经氨酸酶(NEU)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB611Hu02 | 神经氨酸酶(NEU)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB611Hu01 | 神经氨酸酶(NEU)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB611Hu03 | 神经氨酸酶(NEU)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB611Hu01 | 神经氨酸酶(NEU)活性蛋白 | Cell culture; Activity Assays. |
| APB611Hu02 | 神经氨酸酶(NEU)活性蛋白 | Cell culture; Activity Assays. |
| RPB611Hu04 | 神经氨酸酶(NEU)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB611Hu01 | 神经氨酸酶(NEU)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB611Hu02 | 神经氨酸酶(NEU)多克隆抗体 | WB; IHC; ICC; IP. |
| LAB611Hu71 | 神经氨酸酶(NEU)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAB611Hu81 | 神经氨酸酶(NEU)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MAB611Hu21 | 神经氨酸酶(NEU)单克隆抗体 | WB; IHC; ICC; IP. |
| SEB611Hu | 神经氨酸酶(NEU)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| METHODS OF PREVENTING OR TREATING ATHEROSCLEROSIS WITH INHIBITORS OF SPECIFIC ISOENZYMES OF HUMAN NEURAMINIDASE [] |
