对氧磷酶3(PON3)活性蛋白

Active Paraoxonase 3 (PON3)

Serum paraoxonase/lactonase 3

  • 对氧磷酶3(PON3)活性蛋白 产品包装(模拟)
  • 对氧磷酶3(PON3)活性蛋白 产品包装(模拟)
  • 对氧磷酶3(PON3)活性蛋白 Figure . Gene Sequencing (extract)
  • APD178Hu01.jpg Figure. SDS-PAGE
  • 对氧磷酶3(PON3)活性蛋白 Figure. Western Blot; Sample: Recombinant PON3, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Paraoxonase 3 (PON3) is secreted into the bloodstream and associates with high-density lipoprotein (HDL). PON3 also rapidly hydrolyzes lactones and can inhibit the oxidation of low-density lipoprotein, a function that is believed to slow the initiation and progression of atherosclerosis. Besides, Paraoxonase 1 (PON1) has been identified as an interactor of PON3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PON3 and recombinant human PON1. Briefly, PON3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to PON1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-PON3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of PON3 and PON1 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPD178Hu01 对氧磷酶3(PON3)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APD178Hu01 对氧磷酶3(PON3)活性蛋白 Cell culture; Activity Assays.
PAD178Hu01 对氧磷酶3(PON3)多克隆抗体 WB; IHC; ICC; IP.
MAD178Hu21 对氧磷酶3(PON3)单克隆抗体 WB; IHC; ICC; IP.
SED178Hu 对氧磷酶3(PON3)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD178Hu 对氧磷酶3(PON3)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
Journal of Pharmaceutical and Biomedical Analysis Role of esterase mediated hydrolysis of simvastatin in human and rat blood and its impact on pharmacokinetic profiles of simvastatin and its active metabolite in rat [Pubmed: 30776567]
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