纤溶酶原(Plg)活性蛋白

Active Plasminogen (Plg)

PL; Plasmin; Activation peptide; Angiostatin

  • 纤溶酶原(Plg)活性蛋白 产品包装(模拟)
  • 纤溶酶原(Plg)活性蛋白 产品包装(模拟)
  • 纤溶酶原(Plg)活性蛋白 Gene sequencing
  • APB236Ra01.jpg SDS-PAGE图
  • 纤溶酶原(Plg)活性蛋白 Figure. Western Blot; Sample: Recombinant Plg, Rat.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Plasminogen (Plg) can be converted into active plasmin by tissue plasminogen activator (tPA), urokinase plasminogen activator (uPA), kallikrein, and factor XII (Hageman factor). Plasmin can dissolve fibrin blood clots, act on many other processes such as embryonic development, tissue remodeling, inflammation and tumor invasion. Plasmint also can activates collagenases, weakens the walls of the Graafian follicle, cleaves fibrin, fibronectin, thrombospondin, laminin, and von Willebrand factor. Besides, Actin Beta (ACTb) has been identified as an interactor of Plg, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat Plg and recombinant rat ACTb. Briefly, Plg were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ACTb-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-Plg pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of Plg and ACTb was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
RPB236Ra01 纤溶酶原(Plg)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB236Ra01 纤溶酶原(Plg)活性蛋白 Cell culture; Activity Assays.
PAB236Ra01 纤溶酶原(Plg)多克隆抗体 WB; IHC; ICC; IP.
MAB236Ra21 纤溶酶原(Plg)单克隆抗体 WB; IHC; ICC; IP.
MAB236Ra22 纤溶酶原(Plg)单克隆抗体 WB; IHC; ICC; IP.
SEB236Ra 纤溶酶原(Plg)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB236Ra 纤溶酶原(Plg)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSB236Ra01 纤溶酶原(Plg)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
International Journal of Molecular Sciences The Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKC [Mdpi: Source]
J Anim Physiol Anim Nutr (Berl). Improved milk production through PG-PL system by provision of in-house shelter management in lactating Murrah buffaloes during winter season. [pubmed:28084661]
Neurotherapeutics iTRAQ-Based Quantitative Proteomics Reveals the New Evidence Base for Traumatic Brain Injury Treated with Targeted Temperature Management [pubmed:29247448]
Veterinarski Arhiv The influence of a modified micro-environment on stress and milk production through the plasminogen-plasmin system in Murrah buffaloes during the hot-humid … [10.24099:vet.arhiv.170113]
Food Bioscience Reducing antigenicity of ¦Â-lactoglobulin, probiotic properties and safety evaluation of Lactobacillus plantarum AHQ-14 and Lactobacillus bulgaricus BD0390 []
Foods Antigenicity and Safety Evaluation of Lactiplantibacillus plantarum 7-2 Screened to Reduce α-Casein Antigen [Pubmed:35010214]
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