核糖核酸酶P(RNASEP)活性蛋白

Active Ribonuclease P (RNASEP)

Rnase-P; RNASEP1; RPP40; Ribonuclease P/MRP 40kDa Subunit

仅供体外研究使用,不用于临床诊断!警防假冒,可索取进口关税税单及报关单!
  • 核糖核酸酶P(RNASEP)活性蛋白产品包装(模拟)
  • 核糖核酸酶P(RNASEP)活性蛋白产品包装(模拟)
  • APA198Ra01.jpgSDS-PAGE图
  • 核糖核酸酶P(RNASEP)活性蛋白Figure. Western Blot; Sample: Recombinant RNASEP, Rat.
  • Certificate通过ISO 9001、ISO 13485质量体系认证

活性实验

Ribonuclease P is a site specific endonuclease that generates mature tRNAs by catalysing the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. In bacteria, RNase P consists of of two components: a large RNA (about 400 base pairs) encoded by rnpB, and a small protein (119 to 133 amino acids) encoded by rnpA. The RNA moiety of RNase P carries the catalytic activity; the protein component plays an auxiliary, but essential, role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme. The sequence of rnpA is not highly conserved, however there is, in the central part of the protein, a conserved basic region. Besides, Nucleophosmin (NPM) has been identified as an interactor of RNASEP, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat RNASEP and recombinant rat NPM. Briefly, RNASEP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to NPM-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-RNASEP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of RNASEP and NPM was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

赠品

相关产品

编号适用物种:Rattus norvegicus (Rat,大鼠)应用
RPA198Ra01核糖核酸酶P(RNASEP)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APA198Ra01核糖核酸酶P(RNASEP)活性蛋白Cell culture; Activity Assays.
PAA198Ra01核糖核酸酶P(RNASEP)多克隆抗体WB; IHC; ICC; IP.
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