Active Ribonuclease P (RNASEP)
Rnase-P; RNASEP1; RPP40; Ribonuclease P/MRP 40kDa Subunit
- 物种Rattus norvegicus (Rat，大鼠)相同的名称，不同的物种。
- 品牌商CLOUD-CLONE CORP.(CCC,美国)
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 纯度> 97%
- 应用Cell culture; Activity Assays.
- 下载英文说明书 中文说明书
- Figure. Western Blot; Sample: Recombinant RNASEP, Rat.
- 通过ISO 9001、ISO 13485质量体系认证
Ribonuclease P is a site specific endonuclease that generates mature tRNAs by catalysing the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. In bacteria, RNase P consists of of two components: a large RNA (about 400 base pairs) encoded by rnpB, and a small protein (119 to 133 amino acids) encoded by rnpA. The RNA moiety of RNase P carries the catalytic activity; the protein component plays an auxiliary, but essential, role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme. The sequence of rnpA is not highly conserved, however there is, in the central part of the protein, a conserved basic region. Besides, Nucleophosmin (NPM) has been identified as an interactor of RNASEP, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat RNASEP and recombinant rat NPM. Briefly, RNASEP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to NPM-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-RNASEP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of RNASEP and NPM was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
|编号||适用物种：Rattus norvegicus (Rat，大鼠)||应用|
|RPA198Ra01||核糖核酸酶P(RNASEP)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|APA198Ra01||核糖核酸酶P(RNASEP)活性蛋白||Cell culture; Activity Assays.|
|PAA198Ra01||核糖核酸酶P(RNASEP)多克隆抗体||WB; IHC; ICC; IP.|