D-二聚体(D2D)检测试剂盒(化学发光免疫分析法)

CLIA Kit for D-Dimer (D2D)

D 2 Dimer

  • D-二聚体(D2D)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • D-二聚体(D2D)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • D-二聚体(D2D)检测试剂盒(化学发光免疫分析法) 实验结果图
  • CCA506Mu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测D-二聚体(D2D),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的D-二聚体(D2D)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
sodium citrate plasma(n=5) 83-92 87

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的D-二聚体(D2D),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中D-二聚体(D2D)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
sodium citrate plasma(n=5) 96-105% 85-96% 81-98% 89-99%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)50µL,
    加入50µL检测液A(临用前配制);
    37°C温育1小时。
3. 洗板3次;
4. 加检测溶液B100µL,37°C孵育30分钟;
5. 洗板5次;
6. 加底物100µL,37°C孵育10分钟;
7. 读数。

实验原理

本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将D-二聚体(D2D)单克隆抗体包被微孔板,制成固相载体,往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本),待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物,然后加入HRP标记的亲和素,再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的D-二聚体(D2D)呈负相关。用化学发光仪测定相对光单位(RLU),计算样品浓度。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
CEA506Mu D-二聚体(D2D)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
WEA506Mu D-二聚体(D2D)检测试剂盒(酶联免疫吸附试验法,宽范围) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA506Mu D-二聚体(D2D)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA506Mu D-二聚体(D2D)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSA506Mu11 D-二聚体(D2D)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
22 Determinants of the thrombogenic potential of multiwalled carbon nanotubes [ScienceDirect: S0142961211004819]
Journal of Thrombosis and Haemostasis Exogenous Activated Protein C Inhibits the Progression of Diabetic Nephropathy [Pubmed: 22236035]
PLOS ONE Protective Effect of Curcumin on Pulmonary and Cardiovascular Effects Induced by Repeated Exposure to Diesel Exhaust Particles in Mice [PlosOne: Source]
Haemetology Clinical impact of factor V Leiden, prothrombin G20210A, and MTHFR C677T mutations among sickle cell disease patients of Central India [Pubmed: 23992124]
Journal of Trauma and Acute Care Surgery Assessment of coagulopathy, endothelial injury, and inflammation after traumatic brain injury and hemorrhage in a porcine model [Lww:Source]
Journal of Thrombosis and Haemostasis Protein S exacerbates alcoholic hepatitis by stimulating liver natural killer T cells [Pubmed:25399514]
Inflammation.? Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis [Pubmed:25448261]
Medical Science Monitor Impact of β-Adrenoceptor Blockade on Systemic Inflammation and Coagulation Disturbances in Rats with Acute Traumatic Coagulopathy [Pubmed:25676919]
Blood Intraperitoneal administration of activated protein C prevents postsurgical adhesion band formation. [PubMed: 25575539]
Am J Physiol Regul Integr Comp Physiol Procoagulant and Fibrinolytic Activity after Polytrauma in Rat [PubMed: 26632604]
hypertension Research Upregulation of canonical transient receptor potential channel in the pulmonary arterial smooth muscle of a chronic thromboembolic pulmonary hypertension rat model [PubMed: 26155749]
Molecular Nutrition & Food Research Trans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptors [PubMed: 25546502]
JCI Insight. KLF2 and KLF4 control endothelial identity and vascular integrity. [pubmed:28239661]
The American Society of Anesthesiologists Activated protein C drives the hyperfibrinolysis of acute traumatic coagulopathy [pubmed:27841821]
32 Activated TAFI Promotes the Development of Chronic Thromboembolic Pulmonary Hypertension: A Possible Novel Therapeutic Target [pubmed:28289017]
The American Journal of Pathology Inhibition of Cell Apoptosis and Amelioration of Pulmonary Fibrosis by Thrombomodulin [pubmed:28739343]
Atherosclerosis Low dose of alcohol attenuates pro-atherosclerotic activity of thrombin. [pubmed:28923781]
28 Lactadherin Promotes Microvesicle Clearance to Prevent Coagulopathy and Improves Survival of Severe TBI Mice [pubmed:29162596]
Journal of Thrombosis and Thrombolysis Guided longer pulses from a diagnostic ultrasound and intraclot microbubble enhanced catheter-directed thrombolysis in vivo. [pubmed:28417266]
Journal of Investigative Surgery Experimental Evaluation of a New Tissue Factor-Based Topical Hemostat (TT-173) for Treatment of Hepatic Bleeding [Pubmed: 30380352]
Marine Drugs The Inhibitory Effect of Protamine on Platelets is Attenuated by Heparin without Inducing Thrombocytopenia in Rodents [Pubmed: 31533230]
Intensive Care Med Exp Complement factor D is linked to platelet activation in human and rodent sepsis [34396466]
Thromb Res NLRP3 Inflammasome contributes to endotoxin-induced coagulation [Pubmed:35421682]
Experimental and Therapeutic Medicine Construction of a rabbit model with vinorelbine administration via peripherally inserted central catheter and dynamic monitoring of changes in phlebitis and thrombosis [Pubmed:35126715]
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