α2-HS糖蛋白(AHSG)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Alpha-2-Heremans Schmid Glycoprotein (AHSG)

aHSG; HSGa; A2HS; AHS; FETUA; HSG-A2; Fetuin-A; Alpha 2-HS Glycoprotein; Fetuin A; Alpha-2-Z-Globulin; Ba-alpha-2-Glycoprotein

  • α2-HS糖蛋白(AHSG)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • α2-HS糖蛋白(AHSG)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对α2-HS糖蛋白(aHSG)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Bos taurus; Bovine (Cattle,牛) 应用(仅供研究使用,不用于临床诊断!)
RPA178Bo01 α2-HS糖蛋白(AHSG)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA178Bo01 α2-HS糖蛋白(AHSG)多克隆抗体 WB; IHC; ICC; IP.
MAA178Bo21 α2-HS糖蛋白(AHSG)单克隆抗体 WB; IHC; ICC; IP.
SEA178Bo α2-HS糖蛋白(AHSG)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA178Bo α2-HS糖蛋白(AHSG)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSA178Bo01 α2-HS糖蛋白(AHSG)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料
PGA178Bo01 α2-HS糖蛋白(AHSG)引物对 PCR
CGA178Bo01 α2-HS糖蛋白(AHSG)cDNA PCR、蛋白表达等

参考文献

杂志 参考文献
Journal of Proteomics Proteomic analysis reveals oxidative stress response as the main adaptative physiological mechanism in cows under different production systems [ScienceDirect: S1874391912002084]
Journal of Physiology and Biochemistry Curcumin prevents liver fat accumulation and serum fetuin-A increase in rats fed a high-fat diet [PubMed: 23430567]
BioMed Research International Serum Fetuin-A Levels Related with Microalbuminuria in Diet-Induced Obese Rats [PubMed: PMC3591142]
Osteoporosis International Estrogen increases the transcription of human α2-Heremans-Schmid-glycoprotein by an interplay of estrogen receptor α and activator protein-1. [Pubmed: 24504099]
BMC Pharmacology and Toxicology A novel 11β-hydroxysteroid dehydrogenase type1 inhibitor CNX-010-49 improves hyperglycemia, lipid profile and reduces body weight in diet induced obese C57B6/J mice with a potential to provide cardio protective benefits [Pubmed:25098735]
Acta Dermatovenerologica Croatica Relationship Between Serum Fetuin-A Levels and Carotid Intima-media Thickness in Turkish Patients with Mild to Moderate Psoriasis. A Case-control Study [PubMed: 26476900]
Mol Cell Endocrinol Fetuin A promotes lipotoxicity in β cells through the TLR4 signaling pathway and the role of pioglitazone in anti-lipotoxicity. [PubMed: 25986658]
Ann Dermatol Evaluation of Arterial Stiffness in Patients with Beh [PubMed: 26719639]
Endocrine Visceral and subcutaneous adipose tissue express and secrete functional alpha2hsglycoprotein (fetuin a) especially in obesity [pubmed:27738888]
Diabetes Care Targeted Microbiome Intervention by Microencapsulated Delayed-Release Niacin Beneficially Affects Insulin Sensitivity in Humans [pubmed:29212824]
JOURNAL OF ALZHEIMERS DISEASE Acute Anti-Inflammatory Markers ITIH4 and AHSG in Mice Brain of a Novel Alzheimer's Disease Model [Pubmed: 30958362]
Journal of Alzheimer's Disease A New Serum Biomarker Set to Detect Mild Cognitive Impairment and Alzheimer's Disease by Peptidome Technology [Pubmed: 31771070]
Sci Rep Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats [33833309]
J Obstet Gynaecol Res Predıctıve value of pregnancy of follıcular fluıd fetuın‐A and‐B levels ın infertıle women after intra‐cytoplasmic sperm injection [34708901]
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