脂肪酸结合蛋白 4(FABP4)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Fatty Acid Binding Protein 4 (FABP4)

A-FABP; AFABP; AP2; Fatty Acid Binding Protein 4, Adipocyte; Adipocyte Protein 2; Adipocyte-type fatty acid-binding protein

  • 脂肪酸结合蛋白 4(FABP4)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 脂肪酸结合蛋白 4(FABP4)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对脂肪细胞型脂肪酸结合蛋白(FABP4)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Mus musculus (Mouse，小鼠) 应用(仅供研究使用，不用于临床诊断！)
RPB693Mu02 脂肪酸结合蛋白 4(FABP4)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB693Mu01 脂肪酸结合蛋白 4(FABP4)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPB693Mu61 脂肪酸结合蛋白 4(FABP4)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB693Mu01 脂肪酸结合蛋白 4(FABP4)活性蛋白 Cell culture; Activity Assays.
APB693Mu61 脂肪酸结合蛋白 4(FABP4)活性蛋白 Cell culture; Activity Assays.
PAB693Mu06 脂肪酸结合蛋白 4(FABP4)多克隆抗体 WB; IHC; ICC; IP.
PAB693Mu02 脂肪酸结合蛋白 4(FABP4)多克隆抗体 WB; IHC; ICC; IP.
PAB693Mu01 脂肪酸结合蛋白 4(FABP4)多克隆抗体 WB; IHC; ICC; IP.
LAB693Mu71 脂肪酸结合蛋白 4(FABP4)多克隆抗体(生物素标记) WB; IHC; ICC.
LAB693Mu81 脂肪酸结合蛋白 4(FABP4)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
SEB693Mu 脂肪酸结合蛋白 4(FABP4)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB693Mu 脂肪酸结合蛋白 4(FABP4)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB693Mu01 脂肪酸结合蛋白 4(FABP4)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
Clinical and Molecular Hepatology Enhanced A-FABP expression in visceral fat: potential contributor to the progression of NASH [PubMed: 23091808]
journal of Human Hypertension Siesta is associated with reduced systolic blood pressure level and decreased prevalence of hypertension in older adults [PubMed: 26134622]
genetics and molecular research Overexpression of the A-FABP gene facilitates intermuscular fat deposition in transgenic mice [PubMed: 25867423]
Food Chem Cranberries (Oxycoccus quadripetalus) inhibit lipid metabolism and modulate leptin and adiponectin secretion in 3T3-L1 adipocytes [PubMed: 25952883]
Placenta Increased expression of fatty acid binding protein 4 in preeclamptic Placenta and its relevance to preeclampsia [Pubmed:26992681]
Clin Chim Acta. Valproate acid (VPA)-induced dysmetabolic function in clinical and animal studies. [pubmed:28161274]
Oxidative Medicine and Cellular Longevity Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague … [Pubmed:29849871]
Nutrition & Diabetes Lack of pronounced changes in the expression of fatty acid handling proteins in adipose tissue and plasma of morbidly obese humans [Pubmed:29335416]
American Journal of Cancer Research 25-HC promotes hepatocellular carcinoma metastasis through up-regulation of TLR4 dependent FABP4 [Pubmed: 31720079]
J Magn Reson Imaging Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver … [Pubmed:35212074]
cells The Effect of Mineralocorticoid Receptor 3 Antagonists on Anti-Inflammatory and Anti-Fatty Acid Transport Profile in Patients with Heart Failure [Pubmed:35455943]
Bone Research FABP4 secreted by M1-polarized macrophages promotes synovitis and angiogenesis to exacerbate rheumatoid arthritis [Pubmed:35729106]
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