饥饿素(GHRL)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Ghrelin (GHRL)

MTLRP; Growth Hormone-Releasing Peptide

  • 饥饿素(GHRL)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 饥饿素(GHRL)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 饥饿素(GHRL)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品图片
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对饥饿素(GHRL)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

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参考文献

杂志 参考文献
Archives of Oral Biology Expression of ghrelin in human salivary glands and its levels in saliva and serum in Chinese obese children and adolescents [PubMed: 21075359]
Department of Medical Biochemistry and Clinical Biochemistry Presence of adropin, nesfatin-1, apelin-12, ghrelins and salusins peptides in the milk, cheese whey and plasma of dairy cows [ScienceDirect: S0196978113000533]
Journal of Physiology and Pharmacology Postnatal administration of 2-oxoglutaric acid improves articular and growth plate cartilages and bone tissue morphology in pigs prenatally treated with dexamethasone [PubMed: 23211309]
Gene The modifying effects of fish oil on fasting ghrelin mRNA expression in weaned rats [PubMed: 22842192]
Hormone Research in Paediatrics Changes in Ghrelin and Obestatin Levels before and after a Meal in Children with Simple Obesity and Anorexia [Pubmed: 23774845]
Surgical Innovation Gastric supply manipulation to modulate ghrelin production and enhance vascularization to the cardia: proof of the concept in a porcine model [Pubmed:25294792]
Obesity Surgery Embolization of Arterial Gastric Supply in Obesity (EMBARGO): an Endovascular Approach in the Management of Morbid Obesity. Proof of the Concept in the Porcine Model [Pubmed:25511751]
Obes Surg Effect of Sleeve Gastrectomy Plus Side-to-Side Jejunoileal Anastomosis for Type 2 Diabetes Control in an Obese Rat Model [PubMed: 26202420]
Surg Innov Gastric Supply Manipulation to Modulate Ghrelin Production and Enhance Vascularization to the Cardia Proof of the Concept in a Porcine Model [PubMed: 25294792]
Nutrients.  Elevation of Fasting Ghrelin in Healthy Human Subjects Consuming a High-Salt Diet: A Novel Mechanism of Obesity? [Pubmed:27240398]
World Journal of Gastroenterology Effects of sleeve gastrectomy with jejuno-jejunal or jejuno-ileal loop onglycolipid metabolism in diabetic rats. [pubmed:27621579]
Ginekologia Polska Increased serum ghrelin in preeclampsia: is ghrelin a friend or a foe? [pubmed:27321099]
Gynecological Endocrinology The association between serum ghrelin levels and large-for-gestational-age fetuses in patients with gestational diabetes mellitus [pubmed:27791436]
Acta Diabetologica Effect of lifestyle improvement program on the biomarkersof adiposity, inflammation and gut hormones in overweight/obese Asian Indians with prediabetes [pubmed:28620678]
Scandinavian Journal of Clinical and Laboratory Investigation Role of serotonin hormone in weight regain after sleeve gastrectomy [pubmed:29228802]
Slovenian Veterinary Research EFFECT OF Garcinia cambogia EXTRACT ON SERUM LEPTIN, GHRELIN, ADIPONECTIN AND INSULIN LEVELS AND BODY WEIGHTS IN RATS FED WITH HIGH … [Slovetres:Source]
The American Journal of the Medical Sciences The Relations Among Serum Ghrelin, Motilin and Gastric Emptying and Autonomic Function in Autoimmune Gastritis [Pubmed:29753372]
American Journal of Perinatology The Role of Umbilical Cord Blood Concentration of IGF-I, IGF-II, Leptin, Adiponectin, Ghrelin, Resistin, and Visfatin in Fetal Growth [Pubmed: 30282106]
Stem Cells and Development The reparative effects of human adipose-derived mesenchymal stem cells in the chemotherapy damaged thymus [Pubmed: 30511904]
The Journal of Nutrition Grape Seed Proanthocyanidin Affects Lipid Metabolism via Changing Gut Microflora and Enhancing Propionate Production in Weaned Pigs [Pubmed: 31175811]
Arch Physiol Biochem Associations of apelin, leptin, irisin, ghrelin, insulin, glucose levels, and lipid parameters with physical activity during eight weeks of regular exercise training [Pubmed: 31290696]
Mol Med Rep Ghrelin system is involved in improvements in glucose metabolism mediated by hyperbaric oxygen treatment in a streptozotocin‑induced type 1 diabetes mouse … [Pubmed: 32901885]
endocrine Omega-3 offers better hypothalamus protection by decreasing POMC expression and elevating ghrelin hormone: a prospective trial to overcome methotrexate … [Pubmed: 32424681]
plos one Serum leptin level is associated with phase angle in CKD5 patients not undergoing dialysis [Pubmed: 30089154]
Physiological and Histopathological Effects of Electroporation Pulse on Stomach of Rats []
International Journal of Livestock Research Effect of Nutritional Supplementation on Treatment of Anoestrus Swamp Buffalo Cows and Heifers []
iraqi journal of science Relationship Between Orexin-A and Insulin Resistance in Patients with Type 2 Diabetes Mellitus []
Indian Journal of Forensic Medicine & Toxicology Altered Serum Marker of Adipokines Profile in Breast Cancer Women []
Br J Nutr The Association of Pre-Pregnancy BMI On Leptin, Ghrelin, Adiponectin, and IGF-1 In Breast Milk, A Case Control Study [34348809]
Nutrients Nutritional Status, Selected Nutrients Intake and Their Relationship with the Concentration of Ghrelin and Adiponectin in Patients with Diabetic Nephropathy [34959967]
scientific reports Effect of Sleeve Gastrectomy, Roux-En-Y Gastric Bypass, and Ileal Transposition on Myocardial Ischaemia–Reperfusion Injury in Non-Obese Non-Diabetic Rats [34903800]
npj Biofilms and Microbiomes The microbiota-gut-kidney axis mediates host osmoregulation in a small desert mammal [Pubmed:35379849]
Food Hydrocolloids Creating texturally tuneable, low calorie and palatable noodle-like food assemblies via microfluidics []
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