对氧磷酶1(PON1)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Paraoxonase 1 (PON1)

ESA; PON; Esterase A; Serum paraoxonase/arylesterase 1; Aromatic esterase 1; A-esterase 1; Serum aryldialkylphosphatase 1

  • 对氧磷酶1(PON1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 对氧磷酶1(PON1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对对氧磷酶1(PON1)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
RPA243Ra01 对氧磷酶1(PON1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA243Ra03 对氧磷酶1(PON1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA243Ra04 对氧磷酶1(PON1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA243Ra02 对氧磷酶1(PON1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA243Ra01 对氧磷酶1(PON1)多克隆抗体 WB; IHC; ICC; IP.
PAA243Ra02 对氧磷酶1(PON1)多克隆抗体 WB; IHC; ICC; IP.
LAA243Ra71 对氧磷酶1(PON1)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA243Ra51 对氧磷酶1(PON1)多克隆抗体(别藻蓝蛋白标记) WB; IHC; ICC; IF.
MAA243Ra21 对氧磷酶1(PON1)单克隆抗体 WB; IHC; ICC; IP.
SEA243Ra 对氧磷酶1(PON1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA243Ra 对氧磷酶1(PON1)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA243Ra01 对氧磷酶1(PON1)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
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Clinica Chimica Acta Persistent elevation of paraoxonase-1 specific enzyme activity after weight reduction in obese non-diabetic men with metabolic syndrome [PubMed: 21704607]
Journal of Laparoendoscopic & Advanced Surgical Techniques Combined Serum Proteomic and Metabonomic Profiling After Laparoscopic Sleeve Gastrectomy in Children and Adolescents [Wiley: source]
PLoS One. High density lipoprotein structural changes and drug response in lipidomic profiles following the long-term fenofibrate therapy in the FIELD substudy [PubMed: 21887280]
Atherosclerosis Macrophage cholesterol efflux to plasma and HDL in subjects with low and high homocysteine levels: a FIELD substudy [PubMed: 21696738]
Lipids in Health and Disease Beneficial effects of omega-3 fatty acids in the proteome of high-density lipoprotein proteome [PubMed: PMC3480889]
British Journal of Nutrition Extra-virgin olive oil consumption reduces the age-related decrease in HDL and paraoxonase 1 anti-inflammatory activities [Pubmed: 23510814]
Diabetes & Metabolic Syndrome Paraoxonase 1: A better atherosclerotic risk predictor than HDL in type 2 diabetes mellitus [Pubmed: 23680251]
Redox Report Paraoxonase responses to exercise and niacin therapy in men with metabolic syndrome [Pubmed:25180827]
Toxicol Ind Health. Influence of α-tocopherol and α-lipoic acid on bisphenol-A-induced oxidative damage in liver and ovarian tissue of rats [Pubmed:25548375]
RETOS. Nuevas Tendencias en Educación Física, Deporte y Recreación A single session of aerobic exercise influences paraoxonase 1 activity and concentration [Articulo: 345738764042]
PLoS One Plasma Protein Biomarkers of Hepatocellular Carcinoma in HCV-Infected Alcoholic Patients with Cirrhosis [PubMed: 25789864]
Renal Failure Association of lipid profile with serum PON1 concentration in patients with chronic kidney disease [Pubmed:26888625]
Pharmacological reports The Role of PON-1, GR, IL-18, and oxLDL in Depression With and Without Posttraumatic Stress Disorder [science:article]
Clinical and Experimental Hypertension Association of paraoxonase 1 (PON1) gene polymorphisms and concentration with essential hypertension [pubmed:27668323]
Diabetes & Metabolic Syndrome: Clinical Research & Reviews Paraoxonase-1 is a better indicator than HDL of Atherosclerosis-A pilot Study in North Indian population [pubmed:29254890]
Diabetes/Metabolism Research and Reviews A Case-control Study: The Association of Serum Paraoxonase 1 Activity and Concentration with the Development of Type 2 Diabetes Mellitus [pubmed:29156090]
Journal of Chromatography A Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858]
Pharmacological Reports The role of PON-1, GR, IL-18, and OxLDL in depression with and without posttraumatic stress disorder. [pubmed:28623707]
American Journal of Food Science and Health Palm Wine and Orijin Bitters Severed Hyperlipidemia and Immunomodulatory Responses in Atherogenic-diet Fed Male Wistar Rats [ISSN: 2381-7224]
Metabolic brain disease Individuals with autism have higher 8-Iso-PGF2α levels than controls, but no correlation with quantitative assay of Paraoxonase 1 serum levels [pubmed:28808839]
Therapeutic Apheresis And Dialysis Serum Endocan Levels are Associated With Paraoxonase 1 Concentration in Patients With Chronic Kidney Disease [Pubmed:29368426]
Biological Trace Element Research Effect of Aqueous Extract of Glycyrrhiza glabra on the Biochemical Changes Induced by Cadmium Chloride in Rats [Pubmed: 30251228]
GLYCOCONJUGATE JOURNAL Identification a novel clinical biomarker in early diagnosis of human non-small cell lung cancer [Pubmed: 30607521]
Hematology. Differentially expressed plasma proteins of β-thalassemia/hemoglobin E patients in response to curcuminoids/vitamin E antioxidant cocktails [Pubmed: 30661467]
Proteomics Clinical Applications Comparative proteomics analysis of serum proteins in gestational diabetes during early and middle stages of pregnancy [Pubmed: 31162828]
Environment International Toxicity assessment due to prenatal and lactational exposure to lead, cadmium and mercury mixtures [Pubmed: 31639605]
Nutrition, Metabolism and Cardiovascular Diseases Phenotypes and concentration of PON1 in cardiovascular disease: the role of nutrient intake [Pubmed: 31757567]
Environment International Estimating renal and hepatic clearance rates of organophosphate esters in humans: Impacts of intrinsic metabolism and binding affinity with plasma proteins [Pubmed: 31783242]
RESPIRATORY RESEARCH The HDL from septic-ARDS patients with composition changes exacerbates pulmonary endothelial dysfunction and acute lung injury induced by cecal ligation … [Pubmed: 33148285]
CLINICAL RHEUMATOLOGY PON-1 haplotype (-108C> T, L55M, and Q192R) modulates the serum levels and activity PONase promoting an atherogenic lipid profile in rheumatoid arthritis patients [Pubmed: 32556934]
respiratory research The adverse alterations of HDL in quality promote septic ARDS via exacerbated pulmonary endothelial dysfunction []
J Transl Med Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans [33407555]
PLoS One Vitamin D status and systemic redox biomarkers in adults with obesity [30973911]
Oxid Med Cell Longev Repetitions of Strenuous Exercise Consistently Increase Paraoxonase 1 Concentration and Activity in Plasma of Average-Trained Men [34917230]
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