分泌型卷曲相关蛋白1(SFRP1)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Secreted Frizzled Related Protein 1 (SFRP1)

FRP1; FRP; FRP-1; FrzA; SARP2; Secreted apoptosis-related protein 2

  • 分泌型卷曲相关蛋白1(SFRP1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 分泌型卷曲相关蛋白1(SFRP1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对分泌型卷曲相关蛋白1(SFRP1)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Mus musculus (Mouse，小鼠) 应用(仅供研究使用，不用于临床诊断！)
RPF880Mu02 分泌型卷曲相关蛋白1(SFRP1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPF880Mu01 分泌型卷曲相关蛋白1(SFRP1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAF880Mu01 分泌型卷曲相关蛋白1(SFRP1)多克隆抗体 WB; IHC; ICC; IP.
PAF880Mu02 分泌型卷曲相关蛋白1(SFRP1)多克隆抗体 WB; IHC; ICC; IP.
LAF880Mu71 分泌型卷曲相关蛋白1(SFRP1)多克隆抗体(生物素标记) WB; IHC; ICC.
LAF880Mu81 分泌型卷曲相关蛋白1(SFRP1)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
SEF880Mu 分泌型卷曲相关蛋白1(SFRP1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMF880Mu 分泌型卷曲相关蛋白1(SFRP1)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSF880Mu01 分泌型卷曲相关蛋白1(SFRP1)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
BMC Musculoskeletal Disorders Biomarkers and cytokines of bone turnover: extensive evaluation in a cohort of patients with ankylosing spondylitis [PubMed: 23025387]
Exp Cell Res Wnt Inhibition is Dysregulated in Gliomas and its Re-Establishment Inhibits Proliferation and Tumor Sphere Formation [PubMed: 26712519]
BMC Musculoskeletal Disorders Correlation of blood bone turnover biomarkers and Wnt signaling antagonists with AS, DISH,OPLL, and OYL. [pubmed:28153008]
PloS one Effects of exercise training on circulating levels of Dickkpof-1 and secreted frizzled-related protein-1 in breast cancer survivors: A pilot single-blind randomized controlled trial. [pubmed:28178355]
PLoS One Effects of exercise training on circulating levels of Dickkpof-1 and secreted frizzled-related protein-1 in breast cancer survivors: A pilot single-blind randomized controlled trial [10.1371]
Journal of Experimental & Clinical Cancer Research PHF21B overexpression promotes cancer stem cell-like traits in prostate cancer cells by activating the Wnt/β-catenin signaling pathway [pubmed:28645312]
JBMR Plus Low serum levels of DKK2 predict incident low‐impact fracture in older women [Doi: 10.1002/jbm4.10179]
Nature Communications Immunological history governs human stem cell memory CD4 heterogeneity via the Wnt signaling pathway [Pubmed: 32041953]
acta pharmacologica sinica sFRP1 protects H9c2 cardiac myoblasts from doxorubicin-induced apoptosis by inhibiting the Wnt/PCP-JNK pathway [Pubmed: 32238888]
留言咨询