95 KSB966Ra01 | 多配体蛋白聚糖1(SDC1)检测试剂盒DIY材料(酶联免疫吸附试验法) | Rattus norvegicus (Rat，大鼠) USCN(武汉优尔生商贸有限公司)

多配体蛋白聚糖1(SDC1)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Syndecan 1 (SDC1)

CD138; SDC; SYND1; Syndecan Proteoglycan 1

  • 多配体蛋白聚糖1(SDC1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 多配体蛋白聚糖1(SDC1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对多配体蛋白聚糖1(SDC1)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
RPB966Ra02 多配体蛋白聚糖1(SDC1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB966Ra01 多配体蛋白聚糖1(SDC1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB966Ra02 多配体蛋白聚糖1(SDC1)多克隆抗体 WB; IHC; ICC; IP.
PAB966Ra01 多配体蛋白聚糖1(SDC1)多克隆抗体 WB; IHC; ICC; IP.
LAB966Ra81 多配体蛋白聚糖1(SDC1)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAB966Ra21 多配体蛋白聚糖1(SDC1)单克隆抗体 WB; IHC; ICC; IP.
SEB966Ra 多配体蛋白聚糖1(SDC1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB966Ra 多配体蛋白聚糖1(SDC1)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB966Ra01 多配体蛋白聚糖1(SDC1)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
Molecular and Cellular Biochemistry Increased level of soluble syndecan-1 in serum correlates with myocardial expression in a rat model of myocardial infarction [SpringerLink: a201w3l18k257462]
Inflammation Sdc1 Overexpression Inhibits the p38 MAPK Pathway and Lessens Fibrotic Ventricular Remodeling in MI Rats [Springer: Source]
Atherosclerosis. Damage of the endothelial glycocalyx in chronic kidney disease [Pubmed:24727235]
Journal of Trauma and Acute Care Surgery Effect of valproic acid and injury on lesion size and endothelial glycocalyx shedding in a rodent model of isolated traumatic brain injury [Pubmed:25058256]
Shock Impairment of the Endothelial Glycocalyx in Cardiogenic Shock and its Prognostic Relevance [Pubmed:25692257]
Mediators Inflamm Dexamethasone Suppressed LPS-Induced Matrix Metalloproteinase and Its Effect on Endothelial Glycocalyx Shedding [PubMed: 26199464]
Transplantation Platelet-Derived Growth Factor-B Protects Rat Cardiac Allografts From Ischemia-reperfusion Injury. [PubMed: 26371596]
Clinical and Experimental Nephrology Dual antiplatelet and anticoagulant APAC prevents experimental ischemia–reperfusion-induced acute kidney injury [pubmed:27405618]
Biomarkers in Medicine Endothelial glycocalyx layer shedding following lung resection [pubmed:27643669]
Danish National RESEARCH DATABASE Resuscitation with Pooled and Pathogen-Reduced Plasma Attenuates the Increase in Brain Water Content following Traumatic Brain Injury and Hemorrhagic Shock in Rats [10.1089]
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British Journal of Anaesthesia Volume kinetics of Ringer's lactate solution in acute inflammatory disease [10.1016:j.bja.2018.04.023]
Arteriosclerosis, Thrombosis, and Vascular Biology MPO (myeloperoxidase) reduces endothelial glycocalyx thickness dependent on its cationic charge [Pubmed:29903730]
Shock Plasma resuscitation improved survival in a cecal ligation and puncture rat model of sepsis [Pubmed:28591008]
Shock Experimental models of endotheliopathy: impact of shock severity [Pubmed:28697004]
Journal of Trauma and Acute Care Surgery Effects of propranolol and clonidine on brain edema, blood-brain barrier permeability, and endothelial glycocalyx disruption after fluid percussion brain injury in the rat [Pubmed:28930945]
The Endothelial Glycocalyx in the Peritoneal Microcirculation of Rats with Chronic Kidney Failure Exposed to Dialysis Solutions [173015_06.pdf]
Journal of Surgical Research Plasma ameliorates endothelial dysfunction in burn injury [Doi: 10.1016/j.jss.2018.08.027]
Journal of Anesthesia Dexmedetomidine preserves the endothelial glycocalyx and improves survival in a rat heatstroke model [Pubmed: 30374889]
Anaesthesia Postoperative microcirculatory perfusion and endothelial glycocalyx shedding following cardiac surgery with cardiopulmonary bypass [Pubmed: 30687934]
Journal of Cardiothoracic and Vascular Anesthesial Microvascular alterations during cardiac surgery using a heparin or phosphorylcholine coated circuit [Pubmed: 31787433]
Hepatobiliary & Pancreatic Diseases International Increased syndecan-1 and glypican-3 predict poor perinatal outcome and treatment resistance in intrahepatic cholestasis: Syndecan-1, glypican-3 and ICP [Pubmed: 31919038]
Heparin Binding Protein and Endothelial Glycocalyx Markers in Severe COVID-19–A Prospective Observational Cohort Study []
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Newly Developed Recombinant Antithrombin Protects the Endothelial Glycocalyx in an Endotoxin-Induced Rat Model of Sepsis [33375342]
Microvasc Res Effect of liraglutide on microcirculation in rat model with absolute insulin deficiency [34119534]
J Gerontol A Biol Sci Med Sci The effect of pre-operative methylprednisolone on postoperative delirium in elderly patients undergoing gastrointestinal surgery: a randomized, double-blind, placebo … [34423832]
Shock Inhalation of 2% hydrogen improves survival rate and attenuates shedding of vascular endothelial glycocalyx in rats with heat stroke [34524269]
Exp Anim Dexmedetomidine suppresses serum syndecan-1 elevation and improves survival in a rat hemorrhagic shock model [Pubmed:35110424]
Journal of Emergency Medicine Resuscitation of hemorrhagic shock using normal saline does not damage the glycocalyx in the immediate resuscitation phase []
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