晚期糖基化终末产物(AGE)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Advanced Glycation End Product (AGE)
AGEs; Advanced Glycation End Products
- 编号CEB353Ge
- 物种Pan-species (General，通用) 相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长2h
- 检测范围98.8-8,000ng/mL
- 灵敏度最小可检测剂量小于等于38.2ng/mL.
- 样本类型serum, plasma and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2985 ¥ 4264 ¥ 19186 ¥ 36241 ¥ 298452
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
实验结果图
-
标准曲线图
-
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测晚期糖基化终末产物(AGE)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的晚期糖基化终末产物(AGE)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 84-93 | 89 |
| EDTA plasma(n=5) | 85-95 | 91 |
| heparin plasma(n=5) | 87-103 | 98 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的晚期糖基化终末产物(AGE)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中晚期糖基化终末产物(AGE)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-101% | 79-98% | 98-105% | 90-102% |
| EDTA plasma(n=5) | 99-105% | 80-101% | 81-90% | 95-104% |
| heparin plasma(n=5) | 82-98% | 89-101% | 87-103% | 80-95% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）50µL，
加入50µL检测液A(临用前配制);
37°C温育1小时。
3. 洗板3次；
4. 加检测溶液B100µL，37°C孵育30分钟；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分钟；
7. 加终止液50µL，立即450nm读数。
实验原理
本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将晚期糖基化终末产物(AGE)单克隆抗体包被微孔板，制成固相载体，往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本)，待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物，然后加入HRP标记的亲和素，经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。待测标本浓度越高，标记抗原和抗体的结合就越受到抑制，显色愈浅。显色的深浅与酶量呈正相关，而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度（O.D.值），计算样品浓度。
相关产品
| 编号 | 适用物种：Pan-species (General，通用) | 应用(仅供研究使用，不用于临床诊断！) |
| NPB353Ge01 | 晚期糖基化终末产物(AGE)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB353Ge01 | 晚期糖基化终末产物(AGE)活性蛋白 | Cell culture; Activity Assays. |
| PAB353Ge01 | 晚期糖基化终末产物(AGE)多克隆抗体 | WB; IHC; ICC; IP. |
| LAB353Ge71 | 晚期糖基化终末产物(AGE)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAB353Ge81 | 晚期糖基化终末产物(AGE)多克隆抗体(异硫氰酸荧光素标记) | IHC; ICC;ELISA |
| MAB353Ge21 | 晚期糖基化终末产物(AGE)单克隆抗体 | WB; IHC; ICC; IP. |
| HEB353Ge | 晚期糖基化终末产物(AGE)检测试剂盒(酶联免疫吸附试验法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEB353Ge | 晚期糖基化终末产物(AGE)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB353Ge | 晚期糖基化终末产物(AGE)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSB353Ge11 | 晚期糖基化终末产物(AGE)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Human Immunology | Threshold serum concentrations of tumour necrosis factor alpha (TNFα) as a potential marker of the presence of microangiopathy in children and adolescents with type 1 diabetes mellitus (T1DM) [PubMed: S0198885912005642] |
| Experimental Dermatology | A long-standing hyperglycaemic condition impairs skin barrier by accelerating skin ageing process [PubMed: 22017743] |
| J Hum Nutr Diet. | Daily intake of vitamin D- or calcium-vitamin D-fortified Persian yogurt drink (doogh) attenuates diabetes-induced oxidative stress: evidence for antioxidative properties of vitamin D. [Pubmed: 23829785] |
| Gene | Association of RAGE gene polymorphism with circulating AGEs level and paraoxonase activity in relation to macro-vascular complications in Indian type 2 diabetes mellitus patients. [Pubmed: 23721855] |
| Gynecol Endocrinol. | Lipoprotein-associated phospholipase A2 and AGEs are associated with cardiovascular risk factors in women with history of gestational diabetes mellitus. [Pubmed:24397392] |
| Microvasc Res. | Role of advanced glycation end product (AGE)-induced receptor (RAGE) expression in diabetic vascular complications [Pubmed:24984291] |
| Free Radic Res. | Nitroxides prevent protein glycoxidation in vitro. [Pubmed:25363554] |
| Sleep and Breathing | Serum advanced glycation end products are associated with insulin resistance in male nondiabetic patients with obstructive sleep apnea [Pubmed:25566941] |
| Chem Biol Interact | Ascorbic acid and protein glycation [PubMed: 26163454] |
| Osteoarthritis Cartilage | Establishment of a rabbit model to study the influence of advanced glycation end products accumulation on osteoarthritis and the protective effect of pioglitazone [PubMed: 26321377] |
| journal of human hypertension | Relationship between serum levels of endogenous secretory RAGE and blood pressure in male nondiabetic patients with obstructive sleep apnea [PubMed: 25994997] |
| Redox Biol. | Glycation of bovine serum albumin by ascorbate [PubMed: 26202868] |
| J Oral Pathol Med | Impact of morbid obesity and bariatric surgery on antioxidant/oxidant balance of the unstimulated and stimulated human saliva [PubMed: 26608886] |
| J Agric Food Chem | Inhibition of Advanced Glycation Endproduct Formation by Lotus Seedpod Oligomeric Procyanidins through RAGE-MAPK Signaling and NF-κB Activation in High-Fat-Diet Rats. [PubMed: 26207852] |
| Kineziologija | ADVANCED GLYCATION END PRODUCTS'RESPONSE TO RESISTANCE TRAINING IN POSTMENOPAUSAL WOMEN WITH TYPE II DIABETES [140251: ] |
| Food Funct. | Effects of edible bird's nest on hippocampal and cortical neurodegeneration in ovariectomized rats [PubMed: 25920003] |
| Molecular and Cellular Endocrinology | Beneficial effects of urine-derived stem cells on fibrosis and apoptosis of myocardial, glomerular and bladder cells [Pubmed:26952874] |
| Advances in Experimental Medicine and Biology | Effects of S-Nitroso-N-Acetyl-Penicillamine (SNAP) on Inflammation, Lung Tissue Apoptosis and iNOS Activity in a Rabbit Model of Acute Lung Injury [Pubmed:27334732] |
| Eur J Vasc Endovasc Surg | The Receptor for Advanced Glycation End Products (Rage) and Its Ligands in Plasma and InfrainCavia (Guinea pig )inal Bypass Vein [Pubmed:26905625] |
| molecular and cellular endocrinology | Berberine exerts renoprotective effects by regulating the AGEs-RAGE signaling pathway in mesangial cells during diabetic nephropathy. [pubmed:28087385] |
| cellular physiology and biochemistry | Inhibition of Methylglyoxal-Induced AGEs/RAGE Expression Contributes to Dermal Protection by N-Acetyl-L-Cysteine. [pubmed:28214842] |
| General Physiology and Biophysics | Glutathione is the main endogenous inhibitor of protein glycation. [pubmed:28218612] |
| Osteoarthritis and Cartilage | Establishment of a rabbit model to study the influence of advanced glycation end productsaccumulation on osteoarthritis and the protective effect of pioglitazone. [pubmed:26321377] |
| Scientific Reports | Advanced glycation end products promote VEGF expression and thus choroidal neovascularization via Cyr61-PI3K/AKT signaling pathway [pubmed:29097668] |
| INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | Lychee seed extract protects against neuronal injury and improves cognitive function in rats with type II diabetes mellitus with cognitive impairment [pubmed:29138799] |
| 台湾农业研究 | (台灣農業研究67 (4): 355-364) 香菇柄水萃物改善糖尿病降低阿茲海默氏症罹病風險初探 [] |
| Biological Trace Element Research | Altered Mineral Metabolism and Disequilibrium Between Calcification Promoters and Inhibitors in Chronic Hemodialysis Patients [Pubmed: 30847765] |
| International journal of molecular sciences | Cellular Metabolomics Reveal the Mechanism Underlying the Anti-Atherosclerotic Effects of Aspirin Eugenol Ester on Vascular Endothelial Dysfunction [Pubmed: 31261711] |
| Evidence-Based Complementary and Alternative Medicine | Fushiming Capsule Attenuates Diabetic Rat Retina Damage via Antioxidation and Anti-Inflammation [] |
| Journal of Alzheimers Disease | Effect of Advanced Glycation End Products on the Progression of Alzheimer's Disease [Pubmed: 31561370] |
| Life Sciences | Protective effect of vanillin on diabetic nephropathy by decreasing advanced glycation end products in rats [Pubmed: 31759039] |
| Journal of Diabetes Research | Supplementation with Korean Red Ginseng Improves Current Perception Threshold in Korean Type 2 Diabetes Patients: A Randomized, Double-Blind, Placebo … [Pubmed: 32025522] |
| Pharmaceuticals | A New Insight into Meloxicam: Assessment of Antioxidant and Anti-Glycating Activity in In Vitro Studies [Pubmed: 32927809] |
| Mol Cell Endocrinol | Advanced glycation end products enhance macrophage polarization to the M1 phenotype via the HIF-1α/PDK4 pathway [Pubmed: 32464167] |
| archives of gerontology and geriatrics | The effect of Korean Red Ginseng on sarcopenia biomarkers in type 2 diabetes patients [Pubmed: 32470863] |
| The Role of PPARγ in Hyperglycemia-induced Deleterious Effect on Chondrocytes [] | |
| Biomolecules | Young and Undamaged rMSA Improves the Healthspan and Lifespan of Mice [34439857] |
| Dietary Advanced Glycation End Products Shift the Gut Microbiota Composition and Induce Insulin Resistance in Mice [Pubmed:35210793] |
留言咨询
