血管生成素1(ANGPT1)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Angiopoietin 1 (ANGPT1)

ANG1; AGP1; AGPT; ANG-I

  • 血管生成素1(ANGPT1)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 血管生成素1(ANGPT1)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 血管生成素1(ANGPT1)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA008Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测血管生成素1(ANGPT1)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的血管生成素1(ANGPT1)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 83-95 90
EDTA plasma(n=5) 86-94 90
heparin plasma(n=5) 81-94 88

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的血管生成素1(ANGPT1)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中血管生成素1(ANGPT1)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 79-92% 82-102% 78-103% 83-97%
EDTA plasma(n=5) 87-99% 90-101% 80-93% 88-103%
heparin plasma(n=5) 85-97% 85-99% 83-91% 82-96%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将血管生成素1(ANGPT1)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的血管生成素1(ANGPT1)与连接于固相载体上的抗体结合，然后加入生物素化的血管生成素1(ANGPT1)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的血管生成素1(ANGPT1)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
RPA008Hu01 血管生成素1(ANGPT1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA008Hu02 血管生成素1(ANGPT1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA008Hu03 血管生成素1(ANGPT1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA008Hu01 血管生成素1(ANGPT1)多克隆抗体 WB; IHC; ICC; IP.
PAA008Hu02 血管生成素1(ANGPT1)多克隆抗体 WB; IHC; ICC; IP.
LAA008Hu71 血管生成素1(ANGPT1)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA008Hu23 血管生成素1(ANGPT1)单克隆抗体 WB; IHC; ICC; IP.
MAA008Hu24 血管生成素1(ANGPT1)单克隆抗体 WB; IHC; ICC; IP.
MAA008Hu21 血管生成素1(ANGPT1)单克隆抗体 WB
MAA008Hu22 血管生成素1(ANGPT1)单克隆抗体 WB; IHC; ICC; IP.
LAA008Hu72 血管生成素1(ANGPT1)单克隆抗体(生物素标记) WB; IHC; ICC.
SEA008Hu 血管生成素1(ANGPT1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA008Hu 血管生成素1(ANGPT1)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA008Hu 血管生成素1(ANGPT1)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA008Hu 血管生成素1(ANGPT1)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Theriogenology Evaluation of Changes in Doppler Ultrasonography Indices and Levels of Maternal Serum Angiogenic Factors throughout Pregnancy in Ewes [Science: Article]
J Atheroscler Thromb High Mobility Group Box 1 Promotes Angiogenesis from Bone Marrow-derived Endothelial Progenitor Cells after Myocardial Infarction [PubMed: 25735431]
Archives of Biological Sciences Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrol [publication:299492265_Diabetes-induced_renal_failu]
Am J Transl Res Angiopoietin-1 facilitates recovery of hematopoiesis in radiated mice [Pubmed:27347310]
Clinical research Increased plasma concentrations of interleukin 35 in patients with coronary artery disease [Journal:-19]
Turkish Journal of Medicl Sciences A new angiogenesis prognostic index with VEGFA, PlGF, and angiopoietin 1 predicts survival in patients with advanced gastric cancer [pubmed:28425270]
Sci Rep.  Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis. [pubmed:28276491]
PeerJ  The ratio of serum Angiopoietin-1 to Angiopoietin-2 in patients with cervical cancer is a valuable diagnostic and prognostic biomarker [pubmed:28584715]
Molecular&cellular proteomics Prediction of Response to Sorafenib in Hepatocellular Carcinoma: A Marker Panel by Multiple Reaction Monitoring-Mass Spectrometry [pubmed:28550167]
Tumor Biology Cervical cancer cell–derived angiopoietins promote tumor progression [pubmed:28720059]
Free Patents online RHEUMATOID ARTHRITIS RELATED BIOMARKER [20170350884]
Anesthesiology Vasculotide, an angiopoietin-1 mimetic, restores microcirculatory perfusion and microvascular leakage and decreases fluid resuscitation requirements in … [Pubmed:28968277]
British Journal of Anaesthesia Vasculotide, an angiopoietin-1 mimetic, reduces pulmonary vascular leakage and preserves microcirculatory perfusion during cardiopulmonary bypass in rats [10.1016:j.bja.2018.05.049]
The Journal of Biological Chemistry Toll-like receptor 2 deficiency hyperactivates the FoxO1 transcription factor and induces aging-associated cardiac dysfunction in mice [Pubmed:29929978]
Clinical Significance of Nectin-4 Expression in Metastasis and Angiogenesis for Tumor Relapse [Doi: 10.4143/crt.2018.214]
BIOLOGY OF REPRODUCTION Prostaglandin-mediated effects in early canine corpus luteum: In vivo effects on vascular and immune factors [Pubmed: 30929911]
Scientific Reports Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep Apnea [Pubmed: 31882827]
Biochem Biophys Res Commun Effects of interleukin-6 and tumor necrosis factor-α on the expression of angiogenic and collagenolytic factors in premature and mature adipocytes [Pubmed: 32800538]
J Nutr Biochem Effects of protein malnutrition on haematopoietic regulatory activity of bone marrow mesenchymal stem cells [33705953]
Intensive Care Med Exp The effect of targeting Tie2 on hemorrhagic shock-induced renal perfusion disturbances in rats [33997943]
Gynecol Endocrinol Potential of thrombospondin-1 in treatment of polycystic ovary syndrome rat model: a preliminary study [34282706]
Yonsei Med J RAR-Related Orphan Receptor: An Accelerated Preeclampsia Progression by Activating the JAK/STAT3 Pathway [Pubmed:35619579]
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