白介素12(IL12)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Interleukin 12 (IL12)

p70

  • 白介素12(IL12)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 白介素12(IL12)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 白介素12(IL12)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA111Ga.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测白介素12(IL12),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的白介素12(IL12)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 78-101 82
EDTA plasma(n=5) 79-101 90
heparin plasma(n=5) 79-94 88

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的白介素12(IL12),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中白介素12(IL12)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 89-103% 94-101% 86-96% 95-103%
EDTA plasma(n=5) 79-99% 90-98% 79-98% 95-104%
heparin plasma(n=5) 79-90% 88-103% 87-94% 78-102%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将IL12A抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中IL12与连接于固相载体上的抗体结合,然后加入生物素化的IL12B抗体,在将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的IL12浓度呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Chicken (Gallus,鸡) 应用(仅供研究使用,不用于临床诊断!)
RPA111Ga01 白介素12(IL12)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
SEA111Ga 白介素12(IL12)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA111Ga 白介素12(IL12)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
Journal of Virology Characterization of Simian Immunodeficiency Virus Variants Anatomically Compartmentalized inPlasma and Milk in Chronically Infected African Green Monkeys. [pubmed:]
Cancer Science A Novel Potential Strategy for Enhancing Antigen Presentation of Human Hepatocellular Carcinoma Cells Propagated ex-vivo Using a Ribonuclease Enzyme … [Scholarena:Source]
International Immunopharmacology Switched memory B cells promote alveolar bone damage during periodontitis: An adoptive transfer experiment [Pubmed:30015235]
Oncology Letters Antitumor efficacy of the Runx2‑dendritic cell vaccine in triple‑negative breast cancer in vitro [Pubmed:30127867]
Neuroscience The P2X7 receptor, cathepsin S and fractalkine in the trigeminal subnucleus caudalis signal persistent hypernociception in temporomandibular rat joints [Pubmed: 30248434]
Cancer Biomarkers Soluble LAG3 acts as a potential prognostic marker of gastric cancer and its positive correlation with CD8+ T cell frequency and secretion of IL-12 and INF-γ in … [Pubmed: 30223387]
International Journal of Clinical and Experimental Medicine The relationship between autophagy and apoptosis of lamina propria dendritic cells from the small intestine on the immunodynamics of sepsis mice []
ВЛИЯНИЕ ДНК МЕТИЛТРАНСФЕРАЗЫ 3А И ГИСТОНДЕАЦЕТИЛАЗЫ НА СОДЕРЖАНИЕ В МОНОНУКЛЕАРНЫХ КЛЕТКАХ ЦЕЛЬНОЙ КРОВИ … []
Experimental and Therapeutic Medicine RBP and Alb in neonatal hydronephrosis and its association with IL-12 levels in pregnant women before delivery []
Chemosphere Atmospheric H2S triggers immune damage by activating the TLR-7/MyD88/NF-κB pathway and NLRP3 inflammasome in broiler thymus []
Infection and Drug Resistance Disseminated And Infection Accompanied Sweet's Syndrome In A Patient With Anti-Interferon-γ Autoantibodies: A Case Report. [Pubmed: 31632104]
Biomed Pharmacother Suppression of Fpr2 expression protects against endotoxin-induced acute lung injury by interacting with Nrf2-regulated TAK1 activation [Pubmed: 32106380]
Silkworm pupa oil attenuates acetaminophen‐induced acute liver injury by inhibiting oxidative stress‐mediated NF‐κB signaling [Pubmed: 31993149]
J Vet Med Sci Innate immune response of mammary gland induced by intramammary infusion of Bifidobacterium breve in lactating dairy cows [Pubmed: 33071254]
Batı Karadeniz Tıp Dergisi Vasküler Demans Modelinde Çevresel Zenginleştirmenin Depresyon Benzeri Davranış, Kortikal ve Hipokampal BDNF ve IL-1β Seviyeleri Üzerine Etkisi []
Evaluation of lipopolysaccharide-induced acute lung injury attenuation in mice by Glycyrrhiza glabra []
Life Sci BBG enhances OLT1177-induced NLRP3 inflammasome inactivation by targeting P2X7R/NLRP3 and MyD88/NF-¦ÊB signaling in DSS-induced colitis in rats [33548287]
IUBMB Life HOXA5 induces M2 macrophage polarization to attenuate carotid atherosclerosis by activating MED1 [34117711]
Int J Biol Macromol Establishing novel roles of bifidocin LHA, antibacterial, antibiofilm and immunomodulator against Pseudomonas aeruginosa corneal infection model [34171261]
Am J Transl Res Combined treatment with epigenetic agents enhances anti-tumor activity of T cells by upregulating the ACRBP expression in hepatocellular carcinoma [34377237]
J Periodontol Cytokine profile in serum and gingival crevicular fluid of children with inflammatory bowel disease: A case‐control study [34730850]
Biomedicines Exploring Epithelial–Mesenchymal Transition Signals in Endometriosis Diagnosis and In Vitro Fertilization Outcomes [34829910]
Journal of Nanobiotechnology Biomimetic GBM-targeted drug delivery system boosting ferroptosis for immunotherapy of orthotopic drug-resistant GBM [Pubmed:35351131]
J Nanobiotechnology Cyclosporine A loaded brain targeting nanoparticle to treat cerebral ischemia/reperfusion injury in mice [Pubmed:35658867]
Biomedicines Deletion of Toxoplasma Rhoptry Protein 38 (PruΔrop38) as a Vaccine Candidate for Toxoplasmosis in a Murine Model [Pubmed:35740356]
留言咨询