血管活性肠肽(VIP)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Vasoactive Intestinal Peptide (VIP)

PHM27; Intestinal peptide PHV-42; Peptide histidine valine 42; Peptide histidine methioninamide 27

  • 血管活性肠肽(VIP)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 血管活性肠肽(VIP)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 血管活性肠肽(VIP)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • CEA380Ra.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测血管活性肠肽(VIP),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的血管活性肠肽(VIP)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 86-101 89
EDTA plasma(n=5) 80-101 91
heparin plasma(n=5) 86-94 90

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的血管活性肠肽(VIP),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中血管活性肠肽(VIP)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 78-102% 91-98% 81-88% 90-104%
EDTA plasma(n=5) 93-101% 78-103% 90-97% 80-93%
heparin plasma(n=5) 87-94% 99-105% 83-103% 80-104%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)50µL,
    加入50µL检测液A(临用前配制);
    37°C温育1小时。
3. 洗板3次;
4. 加检测溶液B100µL,37°C孵育30分钟;
5. 洗板5次;
6. 加TMB底物90µL,37°C孵育10-20分钟;
7. 加终止液50µL,立即450nm读数。

实验原理

本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将血管活性肠肽(VIP)单克隆抗体包被微孔板,制成固相载体,往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本),待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物,然后加入HRP标记的亲和素,经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。待测标本浓度越高,标记抗原和抗体的结合就越受到抑制,显色愈浅。显色的深浅与酶量呈正相关,而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA380Ra01 血管活性肠肽(VIP)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA380Ra01 血管活性肠肽(VIP)多克隆抗体 WB; IHC; ICC; IP.
MAA380Ra21 血管活性肠肽(VIP)单克隆抗体 WB; IHC; ICC; IP.
CEA380Ra 血管活性肠肽(VIP)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA380Ra 血管活性肠肽(VIP)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSA380Ra11 血管活性肠肽(VIP)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Clinical Genetics VIP gene variants related to idiopathic pulmonary arterial hypertension in Chinese population [Wiley: source]
Human immunology Low levels of vasoactive intestinal peptide are associated with Chagas disease cardiomyopathy [Pubmed: 23800435]
Headache: The Journal of Head and Face Pain CGRP and VIP Levels as Predictors of Efficacy of Onabotulinumtoxin Type A in Chronic Migraine [Onlinelibrary: head.12372]
World J Gastroenterol Roles of sphincter of Oddi motility and serum vasoactive intestinal peptide, gastrin and cholecystokinin octapeptide [NCBI: PMC4000510]
Cephalalgia? Increased VIP levels in peripheral blood outside migraine attacks as a potential biomarker of cranial parasympathetic activation in chronic migraine [Pubmed:24847167]
J Huazhong Univ Sci Technolog Med Sci. Clinical study of electro-acupuncture treatment with different intensities for functional constipation patients [Pubmed:25318892]
PLOS ONE Aqueous Fraction of Beta vulgaris Ameliorates Hyperglycemia in Diabetic Mice due to Enhanced Glucose Stimulated Insulin Secretion, Mediated by Acetylcholine and GLP-1, and Elevated Glucose Uptake via Increased Membrane Bound GLUT4 Transporters [Pubmed:25647228]
PLOS ONE Aqueous Fraction of [PubMed: 25647228]
Pharmacol Res Oral curcumin has anti-arthritic efficacy through somatostatin generation [PubMed: 25836921]
World J Gastroenterol Effects of sphincter of Oddi motility on the formation of cholesterol gallstones [Pubmed:27350732]
Annals of Allergy, Asthma and Immunology Effect of acupuncture on house dust mite specific IgE, substance P, and symptoms in persistent allergic rhinitis [Pubmed:27156748]
Cephalalgia. Relationship between serum levels of VIP, but not of CGRP, and cranial autonomic parasympathetic symptoms: A study in chronic migraine patients [Pubmed:27250233]
Headache No Change in Interictal PACAP Levels in Peripheral Blood in Women With Chronic Migraine [pubmed:27634731]
Sci Rep. Using the "target constituent removal combined with bioactivity assay" strategy to investigate the optimum arecoline content in charred areca nut. [pubmed:28054652]
BMC Complementary and Alternative Medicine [6]-Gingerol, from Zingiber officinale, potentiates GLP-1 mediated glucose-stimulated insulin secretion pathway in pancreatic β-cells and increases RAB8/RAB10-regulated membrane presentation of GLUT4 transporters in skeletal muscle to improve hyperglycemia in Leprdb/db type 2 diabetic mice [10.1186/s12906-017-1903-0]
Applied Biological Chemistry Positive enhancement of Lactobacillus fermentum HY01 on intestinal movements of mice having constipation [10.1007/s13765-017-0327-3]
Journal of Periodontology Salivary neuropeptides, stress, and periodontitis [Pubmed:28914594]
Evidence-Based Complementary and Alternative Medicine Home-Based Transcutaneous Neuromodulation Improved Constipation via Modulating Gastrointestinal Hormones and Bile Acids [Pubmed:29853946]
Revista do Instituto de Medicina Tropical de São Paulo Vasoactive intestinal peptide degradation might influence Interleukin-17 expression in cardiac chagasic patients [Pubmed: 30365640]
Journal of Veterinary Science Serum Levels of Neuropeptides in Cows with Left Abomasal Displacement [Pubmed: 30562932]
Science of Food and Agriculture Protective Effect of Silkworm Pupa Oil on Hydrochloric Acid/Ethanol‐Induced Gastric Ulcer [Pubmed: 30479041]
MOLECULAR BIOLOGY REPORTS Serum CGRP, VIP, and PACAP usefulness in migraine: a case–control study in chronic migraine patients in real clinical practice [Pubmed: 32951099]
Journal of Cystic Fibrosis Disrupted local innervation results in less VIP expression in CF mice tissues [Pubmed: 32600901]
Biomedical Journal of Scientific & Technical Research Anti-Inflammatory and Pro-Inflammatory Neuropeptides in Dilative Cardiomyopathy are there Relations to Psychophysical Stress? []
Journal of tranditional chinese medical sciences Ganjiang granule regulates cecal microflora and serum biochemical components in a rat model of constipation-predominant irritable bowel syndrome []
J Cyst Fibros VIP reduction in the pancreas of F508del homozygous CF mice and early signs of Cystic Fibrosis Related Diabetes (CFRD) [34034984]
Neurol Sci Significance of serum VIP and PACAP in multiple sclerosis: an exploratory case–control study [34698942]
Endocr J A clinical analysis on functioning pancreatic neuroendocrine tumors (focusing on VIPomas): a single-center experience [Pubmed:35644576]
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