血管活性肠肽(VIP)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Vasoactive Intestinal Peptide (VIP)

PHM27; Intestinal peptide PHV-42; Peptide histidine valine 42; Peptide histidine methioninamide 27

  • 血管活性肠肽(VIP)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 血管活性肠肽(VIP)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 血管活性肠肽(VIP)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • CEA380Gu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测血管活性肠肽(VIP),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的血管活性肠肽(VIP)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 94-102 98
EDTA plasma(n=5) 99-105 102
heparin plasma(n=5) 79-91 82

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的血管活性肠肽(VIP),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中血管活性肠肽(VIP)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 83-102% 81-94% 99-105% 90-105%
EDTA plasma(n=5) 86-101% 88-96% 89-99% 80-102%
heparin plasma(n=5) 80-90% 92-105% 84-102% 78-91%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)50µL,
    加入50µL检测液A(临用前配制);
    37°C温育1小时。
3. 洗板3次;
4. 加检测溶液B100µL,37°C孵育30分钟;
5. 洗板5次;
6. 加TMB底物90µL,37°C孵育10-20分钟;
7. 加终止液50µL,立即450nm读数。

实验原理

本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将血管活性肠肽(VIP)单克隆抗体包被微孔板,制成固相载 体,往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本),待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物,然后加入HRP标记的亲和素,经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。待测标本浓度越高,标记抗原和抗体的结合就越受到抑制,显色愈浅。显色的深浅与酶量呈正相关,而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Cavia (Guinea pig ,豚鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA380Gu01 血管活性肠肽(VIP)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA380Gu01 血管活性肠肽(VIP)多克隆抗体 WB; IHC; ICC; IP.
MAA380Gu21 血管活性肠肽(VIP)单克隆抗体 WB; IHC; ICC; IP.
CEA380Gu 血管活性肠肽(VIP)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA380Gu 血管活性肠肽(VIP)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
Clinical Genetics VIP gene variants related to idiopathic pulmonary arterial hypertension in Chinese population [Wiley: source]
Human immunology Low levels of vasoactive intestinal peptide are associated with Chagas disease cardiomyopathy [Pubmed: 23800435]
Headache: The Journal of Head and Face Pain CGRP and VIP Levels as Predictors of Efficacy of Onabotulinumtoxin Type A in Chronic Migraine [Onlinelibrary: head.12372]
World J Gastroenterol Roles of sphincter of Oddi motility and serum vasoactive intestinal peptide, gastrin and cholecystokinin octapeptide [NCBI: PMC4000510]
Cephalalgia? Increased VIP levels in peripheral blood outside migraine attacks as a potential biomarker of cranial parasympathetic activation in chronic migraine [Pubmed:24847167]
J Huazhong Univ Sci Technolog Med Sci. Clinical study of electro-acupuncture treatment with different intensities for functional constipation patients [Pubmed:25318892]
PLOS ONE Aqueous Fraction of Beta vulgaris Ameliorates Hyperglycemia in Diabetic Mice due to Enhanced Glucose Stimulated Insulin Secretion, Mediated by Acetylcholine and GLP-1, and Elevated Glucose Uptake via Increased Membrane Bound GLUT4 Transporters [Pubmed:25647228]
PLOS ONE Aqueous Fraction of [PubMed: 25647228]
Pharmacol Res Oral curcumin has anti-arthritic efficacy through somatostatin generation [PubMed: 25836921]
World J Gastroenterol Effects of sphincter of Oddi motility on the formation of cholesterol gallstones [Pubmed:27350732]
Annals of Allergy, Asthma and Immunology Effect of acupuncture on house dust mite specific IgE, substance P, and symptoms in persistent allergic rhinitis [Pubmed:27156748]
Cephalalgia. Relationship between serum levels of VIP, but not of CGRP, and cranial autonomic parasympathetic symptoms: A study in chronic migraine patients [Pubmed:27250233]
Headache No Change in Interictal PACAP Levels in Peripheral Blood in Women With Chronic Migraine [pubmed:27634731]
Sci Rep. Using the "target constituent removal combined with bioactivity assay" strategy to investigate the optimum arecoline content in charred areca nut. [pubmed:28054652]
BMC Complementary and Alternative Medicine [6]-Gingerol, from Zingiber officinale, potentiates GLP-1 mediated glucose-stimulated insulin secretion pathway in pancreatic β-cells and increases RAB8/RAB10-regulated membrane presentation of GLUT4 transporters in skeletal muscle to improve hyperglycemia in Leprdb/db type 2 diabetic mice [10.1186/s12906-017-1903-0]
Applied Biological Chemistry Positive enhancement of Lactobacillus fermentum HY01 on intestinal movements of mice having constipation [10.1007/s13765-017-0327-3]
Journal of Periodontology Salivary neuropeptides, stress, and periodontitis [Pubmed:28914594]
Evidence-Based Complementary and Alternative Medicine Home-Based Transcutaneous Neuromodulation Improved Constipation via Modulating Gastrointestinal Hormones and Bile Acids [Pubmed:29853946]
Revista do Instituto de Medicina Tropical de São Paulo Vasoactive intestinal peptide degradation might influence Interleukin-17 expression in cardiac chagasic patients [Pubmed: 30365640]
Journal of Veterinary Science Serum Levels of Neuropeptides in Cows with Left Abomasal Displacement [Pubmed: 30562932]
Science of Food and Agriculture Protective Effect of Silkworm Pupa Oil on Hydrochloric Acid/Ethanol‐Induced Gastric Ulcer [Pubmed: 30479041]
MOLECULAR BIOLOGY REPORTS Serum CGRP, VIP, and PACAP usefulness in migraine: a case–control study in chronic migraine patients in real clinical practice [Pubmed: 32951099]
Journal of Cystic Fibrosis Disrupted local innervation results in less VIP expression in CF mice tissues [Pubmed: 32600901]
Biomedical Journal of Scientific & Technical Research Anti-Inflammatory and Pro-Inflammatory Neuropeptides in Dilative Cardiomyopathy are there Relations to Psychophysical Stress? []
Journal of tranditional chinese medical sciences Ganjiang granule regulates cecal microflora and serum biochemical components in a rat model of constipation-predominant irritable bowel syndrome []
J Cyst Fibros VIP reduction in the pancreas of F508del homozygous CF mice and early signs of Cystic Fibrosis Related Diabetes (CFRD) [34034984]
Neurol Sci Significance of serum VIP and PACAP in multiple sclerosis: an exploratory case–control study [34698942]
Endocr J A clinical analysis on functioning pancreatic neuroendocrine tumors (focusing on VIPomas): a single-center experience [Pubmed:35644576]
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