高尔基磷酸化蛋白2(GOLPH2)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Golgi Phosphoprotein 2 (GOLPH2)

GOLM1; GP73; C9orf155; Golgi Membrane Protein 1; Golgi Protein 73

  • 高尔基磷酸化蛋白2(GOLPH2)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 高尔基磷酸化蛋白2(GOLPH2)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对高尔基蛋白73(GP73)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Mus musculus (Mouse，小鼠) 应用(仅供研究使用，不用于临床诊断！)
RPB668Mu02 高尔基磷酸化蛋白2(GOLPH2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB668Mu01 高尔基磷酸化蛋白2(GOLPH2)活性蛋白 Cell culture; Activity Assays.
RPB668Mu01 高尔基磷酸化蛋白2(GOLPH2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB668Mu02 高尔基磷酸化蛋白2(GOLPH2)多克隆抗体 WB; IHC; ICC; IP.
PAB668Mu01 高尔基磷酸化蛋白2(GOLPH2)多克隆抗体 WB; IHC; ICC; IP.
LAB668Mu81 高尔基磷酸化蛋白2(GOLPH2)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAB668Mu71 高尔基磷酸化蛋白2(GOLPH2)多克隆抗体(生物素标记) WB; IHC; ICC.
SEB668Mu 高尔基磷酸化蛋白2(GOLPH2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB668Mu 高尔基磷酸化蛋白2(GOLPH2)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB668Mu01 高尔基磷酸化蛋白2(GOLPH2)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Molecular & Cellular Proteomics LAMC2: A promising new pancreatic cancer biomarker identified by proteomic analysis of pancreatic adenocarcinoma tissues [Mcponline: 023507]
Afro-Egypt J Infect Endem Dis Diagnostic and Prognostic Validity of Serum Golgi Protein 73 in Egyptian Patients with Hepatocelluar Carcinoma [Ajied: Ajied_System_Files]
Journal of Applied Pharmaceutical Science Diagnostic role of Golgi protein 73 and IL-17 in Egyptian cirrhotic rather than hepatocellular carcinoma patients. [handle:123456789]
Oncology Letters Serum Golgi protein 73 is a prognostic rather than diagnostic marker in hepatocellular carcinoma [pubmed:29113278]
Scientific Reports The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian study [Pubmed: 32198440]
Hepatobiliary & Pancreatic Diseases International Dynamic expression of hepatic GP73 mRNA and protein and circulating GP73 during hepatocytes malignant transformation [Pubmed: 32171652]
Biomolecules Deoxycholic Acid Upregulates Serum Golgi Protein 73 through Activating NF-¦ÊB Pathway and Destroying Golgi Structure in Liver Disease. Biomolecules 2021, 11?¡­ [33540642]
ADVANCED RESEARCHES Search for Effective Serum Tumor Markers for Early Diagnosis of Hepatocellular Carcinoma Associated with Hepatitis C []
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