芳基硫酸酯酶B(ARSB)活性蛋白

Active Arylsulfatase B (ARSB)

ASB; G4S; N-acetylgalactosamine-4-sulfatase

  • 芳基硫酸酯酶B(ARSB)活性蛋白 产品包装(模拟)
  • 芳基硫酸酯酶B(ARSB)活性蛋白 产品包装(模拟)
  • APG610Hu01.jpg Figure. SDS-PAGE
  • 芳基硫酸酯酶B(ARSB)活性蛋白 WB图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Arylsulfatase B (ARSB) is a lysosomal enzyme of the sulfatase family. ARSB hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targetted to the lysozyme. Besides, Plasminogen Activator, Urokinase Receptor (uPAR) has been identified as an interactor of ARSB, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ARSB and recombinant human uPAR. Briefly, ARSB were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to uPAR-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ARSB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of ARSB and uPAR was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
APG610Hu01 芳基硫酸酯酶B(ARSB)活性蛋白 Cell culture; Activity Assays.
RPG610Hu01 芳基硫酸酯酶B(ARSB)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAG610Hu01 芳基硫酸酯酶B(ARSB)多克隆抗体 WB; IHC; ICC; IP.
MAG610Hu21 芳基硫酸酯酶B(ARSB)单克隆抗体 WB; IHC; ICC; IP.
SEG610Hu 芳基硫酸酯酶B(ARSB)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMG610Hu 芳基硫酸酯酶B(ARSB)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
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