碳酸酐酶Ⅰ(CA1)活性蛋白

Active Carbonic Anhydrase I (CA1)

CA-I; CAI; Car1; CAB; Carbonic anhydrase B; Carbonate dehydratase I

  • 碳酸酐酶Ⅰ(CA1)活性蛋白 产品包装(模拟)
  • 碳酸酐酶Ⅰ(CA1)活性蛋白 产品包装(模拟)
  • APA875Mu01.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2  H2O = HCO3-  H , which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA1 is a cytosolic enzyme with the highest levels in erythrocytes and is a very early marker for erythroid differentiation. The activity of recombinant mouse CA1 was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 μL of various concentrations of CA1 (diluted by assay buffer) to 50 µL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37℃ for 5min, then read at a wavelength of 400 nm.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Mus musculus (Mouse，小鼠) 应用(仅供研究使用，不用于临床诊断！)
APA875Mu01 碳酸酐酶Ⅰ(CA1)活性蛋白 Cell culture; Activity Assays.
RPA875Mu01 碳酸酐酶Ⅰ(CA1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA875Mu01 碳酸酐酶Ⅰ(CA1)多克隆抗体 WB; IHC; ICC; IP.
LAA875Mu71 碳酸酐酶Ⅰ(CA1)多克隆抗体(生物素标记) WB; IHC; ICC.

参考文献

杂志 参考文献
PLoS ONE Identification of Altered Plasma Proteins by Proteomic Study in Valvular Heart Diseases and the Potential Clinical Significance [PubMed: PMC3754973]
Proteomics Clinical Applications Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patients [Pubmed:26680509]
Am J Trop Med Hyg. Serum Carbonic Anhydrase 1 is a Biomarker for Diagnosis of Human Schistosoma mansoni Infection. [pubmed:28500821]
Journal of Chromatography A Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858]
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