碳酸酐酶Ⅱ(CA2)活性蛋白

Active Carbonic Anhydrase II (CA2)

CA-II; CAII; Car2; CAC; Carbonate dehydratase II; Carbonic anhydrase C

  • 碳酸酐酶Ⅱ(CA2)活性蛋白 产品包装(模拟)
  • 碳酸酐酶Ⅱ(CA2)活性蛋白 产品包装(模拟)
  • APA782Hu01.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2  H2O = HCO3-  H , which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification. The activity of recombinant human CA2 was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 μL of various concentrations of CA2 (diluted by assay buffer) to 50 µL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37℃ for 5min, then read at a wavelength of 400 nm.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
NPA782Hu01 碳酸酐酶Ⅱ(CA2)天然蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA782Hu01 碳酸酐酶Ⅱ(CA2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA782Hu02 碳酸酐酶Ⅱ(CA2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA782Hu04 碳酸酐酶Ⅱ(CA2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA782Hu01 碳酸酐酶Ⅱ(CA2)活性蛋白 Cell culture; Activity Assays.
PAA782Hu01 碳酸酐酶Ⅱ(CA2)多克隆抗体 WB; IHC; ICC; IP.
PAA782Hu04 碳酸酐酶Ⅱ(CA2)多克隆抗体 WB; IHC; ICC; IP.
PAA782Hu03 碳酸酐酶Ⅱ(CA2)多克隆抗体 WB; IHC; ICC; IP.
PAA782Hu02 碳酸酐酶Ⅱ(CA2)多克隆抗体 WB; IHC; ICC; IP.
LAA782Hu71 碳酸酐酶Ⅱ(CA2)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA782Hu22 碳酸酐酶Ⅱ(CA2)单克隆抗体 WB; IHC; ICC; IP.
SEA782Hu 碳酸酐酶Ⅱ(CA2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA782Hu 碳酸酐酶Ⅱ(CA2)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA782Hu01 碳酸酐酶Ⅱ(CA2)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Proteomics Clin Appl.  Identification of potential breast cancer markers in nipple discharge by protein profile analysis using two-dimensional nano-liquid chromatography/nanoelectrospray ionization-mass spectrometry. [Pubmed:26970563]
PROTEOMICS - Clinical Applications Comparative mass spectrometric and immunoassay-based proteome analysis in serum ofDuchenne muscular dystrophy patients. [pubmed:26680509]
Cell & Molecular Biology Identification of potential breast cancer markers in nipple discharge by protein profile analysis using two‐dimensional nano‐liquid chromatography/nanoelectrospray … [doi:10.1002]
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