碳酸酐酶Ⅳ(CA4)活性蛋白

Active Carbonic Anhydrase IV (CA4)

CA-IV; CAIV; Car4; RP17; Retinitis Pigmentosa 17(Autosomal Dominant); Carbonate dehydratase IV

  • 碳酸酐酶Ⅳ(CA4)活性蛋白 产品包装(模拟)
  • 碳酸酐酶Ⅳ(CA4)活性蛋白 产品包装(模拟)
  • APD071Mu01.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2  H2O = HCO3-  H , which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA4 is a GPIanchored membrane enzyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and of proximal renal tubules. It functions as the principal CO2 taste sensor. The activity of recombinant mouse CA4 was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 μL of various concentrations of CA4 (diluted by assay buffer) to 50 µL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37℃ for 5min, then read at a wavelength of 400 nm.

One unit of enzyme activity is defined as the 1 μg of enzyme required to convert 1 pmol of 4-Nitrophenyl acetate to 4-Nitrophenol in 1min at 37°C. The specific activity of recombinant mouse CA4 is > 50 pmol/min/µg.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Mus musculus (Mouse，小鼠) 应用(仅供研究使用，不用于临床诊断！)
APD071Mu01 碳酸酐酶Ⅳ(CA4)活性蛋白 Cell culture; Activity Assays.
RPD071Mu01 碳酸酐酶Ⅳ(CA4)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAD071Mu01 碳酸酐酶Ⅳ(CA4)多克隆抗体 WB; IHC; ICC; IP.
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