CD2分子(CD2)活性蛋白
Active Cluster Of Differentiation 2 (CD2)
LFA2; SRBC; T11; T-Cell Surface Antigen T11/Leu-5; LFA-3 Receptor; Lymphocyte Function Associated Antigen 2; Erythrocyte Receptor; Rosette Receptor; Sheep Red Blood Cell Receptor
- 编号APB576Hu61
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份PBS, pH7.4, containing 5% Trehalose.
- 性状冻干粉
- 纯度> 95%
- 等电点8.9
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1440 ¥ 3600 ¥ 7200 ¥ 21600 ¥ 54000
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Cluster of Differentiation 2 (CD2) is a surface antigen found on all peripheral blood T-cells. CD2 functions as an activator of T lymphocytes and thymocytes. Mature human CD2 consists of a 185 amino acid (aa) extracellular domain (ECD) with an Ig-like V-type and an Ig-like C2 type domain, a 26 aa transmembrane segment, and a 116 aa cytoplasmic tail. Cluster of Differentiation 5 (CD5) has been identified as an interactor of CD2, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human CD2 and recombinant human CD5. Briefly, CD2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to CD5-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CD2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human CD2 and recombinant human CD5 was shown in Figure 1, the EC50 was 1.88 ug/ml.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| EPB576Hu61 | CD2分子(CD2)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB576Hu01 | CD2分子(CD2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB576Hu61 | CD2分子(CD2)活性蛋白 | Cell culture; Activity Assays. |
| RAB576Hu21 | CD2分子(CD2)重组抗体 | WB; IF; ICC; IHC; IP; FCM. |
| FAB576Hu02 | 抗CD2分子(CD2)单克隆抗体 | FCM |
| FAB576Hu04 | 抗CD2分子(CD2)单克隆抗体 | FCM |
| FAB576Hu06 | 抗CD2分子(CD2)单克隆抗体 | FCM |
