内皮细胞特异分子1(ESM1)活性蛋白

Active Endothelial Cell Specific Molecule 1 (ESM1)

Endocan

仅供体外研究使用,不用于临床诊断!请索取进口关税税单及报关单!
  • 内皮细胞特异分子1(ESM1)活性蛋白产品包装(模拟)
  • 内皮细胞特异分子1(ESM1)活性蛋白产品包装(模拟)
  • 内皮细胞特异分子1(ESM1)活性蛋白Gene sequencing
  • APC463Hu01.jpgSDS-PAGE图
  • 内皮细胞特异分子1(ESM1)活性蛋白Figure. Western Blot; Sample: Recombinant ESM1, Human.
  • Certificate通过ISO 9001、ISO 13485质量体系认证

活性实验

Endothelial cell-specific molecule 1 (ESM1) is a proteoglycan secreted by endothelial cells (primarily in the human lung and kidney tissues) and its mRNA expression is regulated by inflammatory cytokines. Endocan expression, which detected in various epithelia and adipocytes has been shown to be upregulated by vascular endothelial growth factor (VEGF), fibroblast growth factor 2 (FGF-2), TNF alpha, IL1 beta, or lipopolysaccharide and downregulated by IFN gamma. Genetically engineered cells overexpressing ESM1 induce tumor formation, implying that ESM1 might be involved in the pathophysiology of tumor growth in vivo. Besides, Lymphocyte Function Associated Antigen 1 Alpha (LFA1a) has been identified as an interactor of ESM1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ESM1 and recombinant human LFA1a. Briefly, ESM1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to LFA1a-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti- ESM1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of ESM1 and LFA1a was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

赠品

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编号适用物种:Homo sapiens (Human,人)应用
APC463Hu01内皮细胞特异分子1(ESM1)活性蛋白Cell culture; Activity Assays.
RPC463Hu01内皮细胞特异分子1(ESM1)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAC463Hu01内皮细胞特异分子1(ESM1)多克隆抗体WB; IHC; ICC; IP.
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MAC463Hu22内皮细胞特异分子1(ESM1)单克隆抗体WB; IHC; ICC; IP.
SEC463Hu内皮细胞特异分子1(ESM1)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
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