成纤维细胞生长因子3(FGF3)活性蛋白

Active Fibroblast Growth Factor 3 (FGF3)

HBGF3; INT2; Heparin-binding growth factor 3; Murine Mammary Tumor Virus Integration Site(v-int-2)Oncogene Homolog; INT-2 Proto-Oncogene Protein; Proto-oncogene Int-2

  • 成纤维细胞生长因子3(FGF3)活性蛋白 产品包装(模拟)
  • 成纤维细胞生长因子3(FGF3)活性蛋白 产品包装(模拟)
  • 成纤维细胞生长因子3(FGF3)活性蛋白 Figure. Gene Sequencing (Extract)
  • APC906Hu01.jpg Figure. SDS-PAGE
  • 成纤维细胞生长因子3(FGF3)活性蛋白 WB图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Fibroblast Growth Factor 3 (FGF3) also known as INT-2 proto-oncogene protein is a member of the fibroblast growth factor family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Besides, Fibroblast Growth Factor Receptor 4 (FGFR4) has been identified as an interactor of FGF3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FGF3 and recombinant human FGFR4. Briefly, biotin-linked FGF3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to FGFR4-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human FGF3 and recombinant human FGFR4 was shown in Figure 1, the EC50 for this effect is 0.52 ug/mL.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPC906Hu01 成纤维细胞生长因子3(FGF3)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APC906Hu01 成纤维细胞生长因子3(FGF3)活性蛋白 Cell culture; Activity Assays.
RPC906Hu02 成纤维细胞生长因子3(FGF3)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAC906Hu01 成纤维细胞生长因子3(FGF3)多克隆抗体 WB; IHC; ICC; IP.
LAC906Hu71 成纤维细胞生长因子3(FGF3)多克隆抗体(生物素标记) WB; IHC; ICC.
MAC906Hu22 成纤维细胞生长因子3(FGF3)单克隆抗体 WB; IHC; ICC; IP.
SEC906Hu 成纤维细胞生长因子3(FGF3)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC906Hu 成纤维细胞生长因子3(FGF3)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMC906Hu 成纤维细胞生长因子3(FGF3)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Mol Med Rep Screening a novel FGF3 antagonist peptide with anti-tumor effects on breast cancer from a phage display library [PubMed: 26323695]
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