白介素28B(IL28B)活性蛋白

Active Interleukin 28B (IL28B)

IFNL3; Interferon,Lambda 3; Cytokine Zcyto22

  • 白介素28B(IL28B)活性蛋白 产品包装(模拟)
  • 白介素28B(IL28B)活性蛋白 产品包装(模拟)
  • APC028Hu01.jpg Figure. SDS-PAGE
  • 白介素28B(IL28B)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. The binding activity of IL28B with IL10Rb.
Interleukin 28B (IL28B) is a cytokine distantly related to type I interferons and the IL-10 family. It has function on antiviral, antitumour and immunomodulatory activities. IL28B also plays a critical role in the antiviral host defense, predominantly in the epithelial tissues and acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Besides, Interleukin 10 Receptor Beta (IL10Rb) has been identified as an interactor of IL28B, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL28B and recombinant human IL10Rb. Briefly, IL28B were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL10Rb-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL28B pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL28B and IL10Rb was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APC028Hu01 白介素28B(IL28B)活性蛋白 Cell culture; Activity Assays.
RPC028Hu01 白介素28B(IL28B)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAC028Hu01 白介素28B(IL28B)多克隆抗体 WB; IHC; ICC; IP.
LAC028Hu81 白介素28B(IL28B)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAC028Hu71 白介素28B(IL28B)多克隆抗体(生物素标记) WB; IHC; ICC.
MAC028Hu22 白介素28B(IL28B)单克隆抗体 WB; IHC; ICC; IP.
HEC028Hu 白介素28B(IL28B)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
SEC028Hu 白介素28B(IL28B)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC028Hu 白介素28B(IL28B)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMC028Hu 白介素28B(IL28B)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Journal of Antimicrobial Chemotherapy Impact of IL28B gene polymorphisms on interferon-λ3 plasma levels during pegylated interferon-α/ribavirin therapy for chronic hepatitis C in patients coinfected with HIV [PubMed: 22294646]
Cytokine Different effects of three interferons L on Toll-like receptor-related gene expression in HepG2 cells. [Pubmed: 24041672]
Hepatology Polymorphisms in melanoma differentiation‐associated gene 5 link protein function to clearance of hepatitis C virus [Pubmed:25130193]
Cytokine. Increased concentration of interferon lambda-3, interferon beta and interleukin-10 in the cerebrospinal fluid of patients with tick-borne encephalitis [Pubmed:25461389]
J Biomed Res.  Impact of IL28B gene polymorphisms rs8099917 and rs12980275 on response to pegylated interferon-α/ribavirin therapy in chronic hepatitis C genotype 4 … [pmc:PMC4726832]
Journal of Biomedical Research Impact of IL28B gene polymorphisms rs8099917 and rs12980275 on response to pegylated interferon-α/ribavirin therapy in chronic hepatitis C genotype 4 patients [PMC4726832]
LUPUS Collagen triple helix repeat containing-1: a novel biomarker associated with disease activity in Systemic lupus erythematosus [Pubmed: 30336754]
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