白介素28B(IL28B)活性蛋白
Active Interleukin 28B (IL28B)
IFNL3; Interferon,Lambda 3; Cytokine Zcyto22
- 编号APC028Hu01
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
- 性状冻干粉
- 纯度> 95%
- 等电点9.0
- 应用Cell culture; Activity Assays.
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- 规格 10µg50µg 200µg 1mg 5mg
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活性实验
Figure. The binding activity of IL28B with IL10Rb.
Interleukin 28B (IL28B) is a cytokine distantly related to type I interferons and the IL-10 family. It has function on antiviral, antitumour and immunomodulatory activities. IL28B also plays a critical role in the antiviral host defense, predominantly in the epithelial tissues and acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Besides, Interleukin 10 Receptor Beta (IL10Rb) has been identified as an interactor of IL28B, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL28B and recombinant human IL10Rb. Briefly, IL28B were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL10Rb-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL28B pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL28B and IL10Rb was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
APC028Hu01 | 白介素28B(IL28B)活性蛋白 | Cell culture; Activity Assays. |
RPC028Hu01 | 白介素28B(IL28B)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAC028Hu01 | 白介素28B(IL28B)多克隆抗体 | WB; IHC; ICC; IP. |
LAC028Hu81 | 白介素28B(IL28B)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
LAC028Hu71 | 白介素28B(IL28B)多克隆抗体(生物素标记) | WB; IHC; ICC. |
MAC028Hu22 | 白介素28B(IL28B)单克隆抗体 | WB; IHC; ICC; IP. |
HEC028Hu | 白介素28B(IL28B)检测试剂盒(酶联免疫吸附试验法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SEC028Hu | 白介素28B(IL28B)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCC028Hu | 白介素28B(IL28B)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMC028Hu | 白介素28B(IL28B)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
杂志 | 参考文献 |
Journal of Antimicrobial Chemotherapy | Impact of IL28B gene polymorphisms on interferon-λ3 plasma levels during pegylated interferon-α/ribavirin therapy for chronic hepatitis C in patients coinfected with HIV [PubMed: 22294646] |
Cytokine | Different effects of three interferons L on Toll-like receptor-related gene expression in HepG2 cells. [Pubmed: 24041672] |
Hepatology | Polymorphisms in melanoma differentiation‐associated gene 5 link protein function to clearance of hepatitis C virus [Pubmed:25130193] |
Cytokine. | Increased concentration of interferon lambda-3, interferon beta and interleukin-10 in the cerebrospinal fluid of patients with tick-borne encephalitis [Pubmed:25461389] |
J Biomed Res. | Impact of IL28B gene polymorphisms rs8099917 and rs12980275 on response to pegylated interferon-α/ribavirin therapy in chronic hepatitis C genotype 4 … [pmc:PMC4726832] |
Journal of Biomedical Research | Impact of IL28B gene polymorphisms rs8099917 and rs12980275 on response to pegylated interferon-α/ribavirin therapy in chronic hepatitis C genotype 4 patients [PMC4726832] |
LUPUS | Collagen triple helix repeat containing-1: a novel biomarker associated with disease activity in Systemic lupus erythematosus [Pubmed: 30336754] |