Janus激酶2(JAK2)活性蛋白

Active Janus Kinase 2 (JAK2)

JTK10; Tyrosine-protein kinase JAK2

  • Janus激酶2(JAK2)活性蛋白 产品包装(模拟)
  • Janus激酶2(JAK2)活性蛋白 产品包装(模拟)
  • Janus激酶2(JAK2)活性蛋白 Gene sequencing
  • APF494Hu01.jpg SDS-PAGE图
  • Janus激酶2(JAK2)活性蛋白 Figure. Western Blot; Sample: Recombinant JAK2, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

JAK2 (Tyrosine-protein kinase JAK2) is a tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. JAK2 is considered to associate with some type I receptors, including EPOR (Erythropoietin receptor), therefore participates in cellular signal transduction. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human JAK2 and recombinant human EPOR. Briefly, JAK2 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL JAK2 were then transferred to EPOR-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-JAK2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of JAK2 and EPOR was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
RPF494Hu01 Janus激酶2(JAK2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APF494Hu01 Janus激酶2(JAK2)活性蛋白 Cell culture; Activity Assays.
PAF494Hu01 Janus激酶2(JAK2)多克隆抗体 WB; IHC; ICC/IF
LAF494Hu71 Janus激酶2(JAK2)多克隆抗体(生物素标记) WB; IHC; ICC.
SEF494Hu Janus激酶2(JAK2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.

参考文献

杂志 参考文献
Scientific Reports Xiaoyaosan exerts anxiolytic-like effects by down-regulating the TNF-α/JAK2-STAT3 pathway in the rat hippocampus. [pubmed:28336920]
Molecular Neurobiology Erythropoietin Rescues Memory Impairment in a Rat Model of Chronic Cerebral Hypoperfusion via the EPO-R/JAK2/STAT5/PI3K/Akt/GSK-3β Pathway [pubmed:28488208]
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