丝氨酸蛋白酶1(PRSS1)活性蛋白
Active Protease, Serine 1 (PRSS1)
TRP1; TRY1; TRY4; TRYP1; Trypsin 1; Cationic Trypsinogen; Beta-trypsin; Serine protease 1; Trypsin I
- 编号APA230Hu02
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 90%
- 等电点7.6
- 应用Cell culture; Activity Assays.
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- 规格 10µg50µg 200µg 1mg 5mg
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活性实验
Human Trypsin 1, encoded by the PRSS1 gene, is also known as cationic trypsinogen. Constituting approximately two-thirds of the total trypsin content in normal pancreatic juice, it is the most abundant trypsin isoform produced by the pancreas. It contains a signal peptide (residues 115), a pro region (residues 1623), and a mature chain (residues 24247). Trypsin 1 is synthesized in the pancreas and secreted into the duodenum lumen, where it is activated by enterokinase. Its major physiologic function is to digest food and to activate other pro-enzyme. Mutations in the PRSS1 gene can cause hereditary pancreatitis. The activity of recombinant human PRSS1 is measured by its ability to cleave a fluorogenic peptide substrate Mca-Arg-Pro-Lys-Pro-Val-Glu-Nval-Trp-Arg-Lys(Dnp)-NH2 in the assay buffer 100 mM Tris, 150 mM NaCl, 10 mM CaCl2, 0.05% (w/v) Brij-35, pH 8.0. The rhPRSS1 is diluted to 200 ug/ml in activation buffer 50 mM Tris, 0.15 M NaCl, 10 mM CaCl2, 0.05% (w/v) Brij-35, pH 7.5, then activated with 1ul Enterokinase at 37 ℃ for 15min. The activated rhPRSS1 is diluted to 3 ug/mL in assay buffer. Loading into a black well plate 50 µL of 3 ug/mL rhPRSS1 and start the reaction by adding 50 µL of 20 µM substrate, with a substrate blank containing 50 µL assay buffer, 50 µL substrate, and no rhPRSS1. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The specific activity of recombinant human PRSS1 is > 600 pmol/min/µg.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
RPA230Hu02 | 丝氨酸蛋白酶1(PRSS1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA230Hu02 | 丝氨酸蛋白酶1(PRSS1)活性蛋白 | Cell culture; Activity Assays. |
RPA230Hu01 | 丝氨酸蛋白酶1(PRSS1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA230Hu01 | 丝氨酸蛋白酶1(PRSS1)多克隆抗体 | WB; IHC; ICC; IP. |
PAA230Hu02 | 丝氨酸蛋白酶1(PRSS1)多克隆抗体 | WB |
MAA230Hu22 | 丝氨酸蛋白酶1(PRSS1)单克隆抗体 | WB; IHC; ICC; IP. |
SEA230Hu | 丝氨酸蛋白酶1(PRSS1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA230Hu | 丝氨酸蛋白酶1(PRSS1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
杂志 | 参考文献 |
annal of medicine and surgery | Laparoscopic procedures impact on mast cell mediators, extracellular matrix and adhesion scoring system in rats [Pubmed: 32963775] |