S100钙结合蛋白A6(S100A6)活性蛋白
Active S100 Calcium Binding Protein A6 (S100A6)
S100-A6; 2A9; 5B10; CABP; CACY; PRA; MLN 4; Calcyclin; Growth factor-inducible protein 2A9; Prolactin receptor-associated protein
- 编号APB769Hu01
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
- 性状冻干粉
- 纯度> 90%
- 等电点5.7
- 应用Cell culture; Activity Assays.
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- 规格 10µg50µg 200µg 1mg 5mg
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活性实验
S100A6 is a calcium-binding protein, which functions as calcium sensor and modulator, contributing to cellular calcium signaling. It is reported that S100B and S100A6 differentially modulate cell survival by Interacting with distinct RAGE (Receptor for Advanced Glycation End Products). Thus a binding ELISA assay was conducted to detect the interaction of S100A6 and RAGE. Briefly, recombinant human S100A6 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RAGE-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-S100A6 mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of S100A6 and RAGE was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
APB769Hu01 | S100钙结合蛋白A6(S100A6)活性蛋白 | Cell culture; Activity Assays. |
RPB769Hu01 | S100钙结合蛋白A6(S100A6)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB769Hu01 | S100钙结合蛋白A6(S100A6)多克隆抗体 | WB,IHC,ICC/IF |
LAB769Hu81 | S100钙结合蛋白A6(S100A6)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
LAB769Hu71 | S100钙结合蛋白A6(S100A6)多克隆抗体(生物素标记) | WB; IHC; ICC. |
MAB769Hu23 | S100钙结合蛋白A6(S100A6)单克隆抗体 | WB; IHC; ICC; IP. |
MAB769Hu22 | S100钙结合蛋白A6(S100A6)单克隆抗体 | WB; ICC/IF |
MAB769Hu21 | S100钙结合蛋白A6(S100A6)单克隆抗体 | IHC; ICC/IF |
MAB769Hu24 | S100钙结合蛋白A6(S100A6)单克隆抗体 | WB; IHC; ICC; IP. |
SEB769Hu | S100钙结合蛋白A6(S100A6)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB769Hu | S100钙结合蛋白A6(S100A6)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
PSB769Hu01 | S100钙结合蛋白A6(S100A6)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSB769Hu01 | S100钙结合蛋白A6(S100A6)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Journal of Proteomics | Tear proteome and protein network analyses reveal a novel pentamarker panel for tear film characterization in dry eye and meibomian gland dysfunction [PubMed: 23201116] |
Tumour Biol | Diagnostic significance of S100A2 and S100A6 levels in sera of patients with non-small cell lung cancer [PubMed: 26361956] |
Clinical and Translational Medicine | Serum levels of S100A6 are unaltered in patients with resectable cholangiocarcinoma [pubmed:27709523] |
Gastroenterology | Tu1938-Micronutrients Assessment in Patients with Cirrhosis [10.1016:S0016-5085(18)33544-3] |
Gastroenterology | Tu1942-PI3K/AKT-Mediated Upregulation of C7 Inhibits Colorectal Cancer Proliferation and Metastasis [10.1016:S0016-5085(18)33544-5] |
Tumor Biology | Proteomics detection of S100A6 in tumor tissue interstitial fluid and evaluation of its potential as a biomarker of cholangiocarcinoma [Pubmed:29629840] |
scandinavian journal of clinical & laboratory investigation | Serum S100A6, S100A8, S100A9 and S100A11 proteins in colorectal neoplasia: results of a single centre prospective study [Pubmed: 31856598] |
CELL BIOCHEMISTRY AND FUNCTION | S100A6 represses Calu©\6 lung cancer cells growth via inhibiting cell proliferation, migration, invasion and enhancing apoptosis [34008212] |
MOLECULAR VISION | Quantification of a panel for dry-eye protein biomarkers in tears: A comparative pilot study using standard ELISA and customized microarrays [34012227] |