细胞因子信号转导抑制因子3(SOCS3)活性蛋白

Active Suppressors Of Cytokine Signaling 3 (SOCS3)

ATOD4; CIS3; Cish3; MGC71791; SOCS-3; SSI-3; SSI3; Cytokine-inducible SH2 protein 3; STAT-induced STAT inhibitor 3

仅供体外研究使用,不用于临床诊断!警防假冒,可索取进口关税税单及报关单!
  • 细胞因子信号转导抑制因子3(SOCS3)活性蛋白产品包装(模拟)
  • 细胞因子信号转导抑制因子3(SOCS3)活性蛋白产品包装(模拟)
  • APB684Mu01.jpgSDS-PAGE图
  • 细胞因子信号转导抑制因子3(SOCS3)活性蛋白Figure. Western Blot; Sample: Recombinant SOCS3, Mouse.
  • Certificate通过ISO 9001、ISO 13485质量体系认证

活性实验

Suppressor of cytokine signaling 3 (SOCS3) is a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family that negatively regulates cytokine signal transduction. SOCS3 is feedback inhibitors of the Janus kinase (JAK) and signal transducer and activator of transcription (STAT) signaling pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. SOCS3 also can bind to JAK2 kinase inhibits its kinase activity and nhibits insulin signaling in adipose tissue and the liver. Besides, Glycoprotein 130 (gp130) has been identified as an interactor of SOCS3, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse SOCS3 and recombinant mouse gp130. Briefly, SOCS3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to SOCS3-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-SOCS3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of SOCS3 and gp130 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

赠品

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编号适用物种:Mus musculus (Mouse,小鼠)应用
RPB684Mu02细胞因子信号转导抑制因子3(SOCS3)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
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APB684Mu01细胞因子信号转导抑制因子3(SOCS3)活性蛋白Cell culture; Activity Assays.
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