叉头框蛋白O1(FOXO1)多克隆抗体
Polyclonal Antibody to Forkhead Box Protein O1 (FOXO1)
FOX-O1; FKH1; FKHR; FOXO1A; Forkhead Homolog In Rhabdomyosarcoma
- 编号PAA764Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 来源多抗制备
- 宿主兔
- 效价-
- Ig类型 IgG
- 纯化方式抗原特异性亲和纯化
- 标记物无标记物
- 免疫原 RPA764Hu01-叉头框蛋白O1(FOXO1)重组蛋白
- 缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
- 性状液体
- 浓度0.25mg/ml
- 且适物种Mus musculus (Mouse，小鼠)
- 应用WB; IHC; IF; FCM
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 569 ¥ 1327 ¥ 1896 ¥ 4740 ¥ 18960
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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DAB staining on IHC-P; Samples: Human Esophagus Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human FOXO1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Stomach cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human FOXO1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Esophagus cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human FOXO1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Cerebrum Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human FOXO1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;
Samples: Human HepG2 cell;
Primary Ab: 20μg/ml Rabbit Anti-Human FOXO1 Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
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Figure. Western Blot; Sample: Recombinant FOXO1, Human.
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Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human FOXO1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human HepG2 cell lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human FOXO1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Liver lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human FOXO1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human HepG2 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human FOXO1 Polyclonal Antibody (Catalog PAA764Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对FOXO1的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别FOXO1。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence:5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
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相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA764Hu01 | 叉头框蛋白O1(FOXO1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA764Hu01 | 叉头框蛋白O1(FOXO1)多克隆抗体 | WB; IHC; IF; FCM |
| MAA764Hu22 | 叉头框蛋白O1(FOXO1)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA764Hu | 叉头框蛋白O1(FOXO1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA764Hu | 叉头框蛋白O1(FOXO1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Biogerontology | The role of melatonin, sirtuin2 and FoXO1 transcription factor in the aging process of colon in male rats [Pubmed:25430724] |
| PLoS One. | Renoprotective Effect of Lactoferrin against Chromium-Induced Acute Kidney Injury in Rats: Involvement of IL-18 and IGF-1 Inhibition [Pubmed:26990190] |
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