补体因子H(CFH)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Complement Factor H (CFH)
CF-H; FH; FHL1; ARMD4; ARMS1; CFHL3; HF1; HF2; HUS; H Factor 2; Age-Related Maculopathy Susceptibility 1; Adrenomedullin binding protein
- 编号KSA635Ra11
- 物种Rattus norvegicus (Rat,大鼠) 相同的名称,不同的物种。
- 试剂内容 包被抗体、生物素标记的竞争物、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma and other biological fluids.
- 可适用原理竞争抑制酶联免疫吸附试验法测抗原
- 可检测范围12.35-1000ng/mL
- 可适灵敏度5.77ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7387 ¥ 12312 ¥ 22162 ¥ 43092 ¥ 73872
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特异性
试剂盒中的抗体对补体因子H(CFH)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Rattus norvegicus (Rat,大鼠) | 应用(仅供研究使用,不用于临床诊断!) |
RPA635Ra01 | 补体因子H(CFH)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA635Ra01 | 补体因子H(CFH)多克隆抗体 | WB; IHC; ICC; IP. |
MAA635Ra21 | 补体因子H(CFH)单克隆抗体 | WB; IHC |
MAA635Ra24 | 补体因子H(CFH)单克隆抗体 | WB; IHC; ICC; IP. |
MAA635Ra26 | 补体因子H(CFH)单克隆抗体 | WB; IHC; ICC; IP. |
LAA635Ra74 | 补体因子H(CFH)单克隆抗体(生物素标记) | WB; IHC; ICC. |
LAA635Ra76 | 补体因子H(CFH)单克隆抗体(生物素标记) | WB; IHC; ICC. |
SEA635Ra | 补体因子H(CFH)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CEA635Ra | 补体因子H(CFH)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA635Ra | 补体因子H(CFH)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
PSA635Ra01 | 补体因子H(CFH)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSA635Ra11 | 补体因子H(CFH)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Pediatr Int | Atypical hemolytic uremic syndrome: Korean pediatric series [PubMed: 25443527] |
Hernia | Circulating matrix metalloproteinases and procollagen propeptides in inguinal hernia [Pubmed:29484522] |
Mikrochim Acta | Fluorescent silver nanoclusters as antibody label in a competitive immunoassay for the complement factor H [Pubmed: 31187271] |
Diagnostics | Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J [Pubmed: 32585848] |
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | The importance of sialic acid, pH and ion concentration on the interaction of uromodulin and complement factor H [33788378] |
Front Immunol | A Family Affair: Addressing the Challenges of Factor H and the Related Proteins [33868311] |
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