补体因子H(CFH)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Complement Factor H (CFH)

CF-H; FH; FHL1; ARMD4; ARMS1; CFHL3; HF1; HF2; HUS; H Factor 2; Age-Related Maculopathy Susceptibility 1; Adrenomedullin binding protein

  • 补体因子H(CFH)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 补体因子H(CFH)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对补体因子H(CFH)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

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参考文献

杂志 参考文献
Pediatr Int Atypical hemolytic uremic syndrome: Korean pediatric series [PubMed: 25443527]
Hernia Circulating matrix metalloproteinases and procollagen propeptides in inguinal hernia [Pubmed:29484522]
Mikrochim Acta Fluorescent silver nanoclusters as antibody label in a competitive immunoassay for the complement factor H [Pubmed: 31187271]
Diagnostics Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J [Pubmed: 32585848]
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE The importance of sialic acid, pH and ion concentration on the interaction of uromodulin and complement factor H [33788378]
Front Immunol A Family Affair: Addressing the Challenges of Factor H and the Related Proteins [33868311]
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