半胱氨酸蛋白酶抑制剂B(CSTB)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Cystatin B (CSTB)

Cys-B; CST6; EPM1; PME; STFB; CPI-B; Stefin B; Liver thiol proteinase inhibitor

  • 半胱氨酸蛋白酶抑制剂B(CSTB)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 半胱氨酸蛋白酶抑制剂B(CSTB)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对半胱氨酸蛋白酶抑制剂B(CSTB)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPJ322Mu01 半胱氨酸蛋白酶抑制剂B(CSTB)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAJ322Mu01 半胱氨酸蛋白酶抑制剂B(CSTB)多克隆抗体 WB; IHC; ICC; IP.
LAJ322Mu71 半胱氨酸蛋白酶抑制剂B(CSTB)多克隆抗体(生物素标记) WB; IHC; ICC.
KSJ322Mu01 半胱氨酸蛋白酶抑制剂B(CSTB)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
AIDS. Cathepsin B and cystatin B in HIV-seropositive women are associated with infection and HIV-1-associated neurocognitive disorders [PubMed: 23291538]
International Journal of Circumpolar Health Evaluation of serum procathepsin B, cystatin B and cystatin C as possible biomarkers of ovarian cancer [PubMed: PMC3754495]
PLoS One. Dysregulation of Macrophage-Secreted Cathepsin B Contributes to HIV-1-Linked Neuronal Apoptosis [PubMed: PMC3365072]
Journal of Neuroimmune Pharmacology Cocaine potentiates cathepsin B secretion and neuronal apoptosis from HIV-infected macrophages [Pubmed:25209871]
European Journal of Cancer Supplements A91: Endogenous inhibitors of cysteine proteases and preform of cathepsin B in cancer of reproductive system [Science: Article]
Annals of Clinical Biochemistry Diagnostic specificity and sensitivity of PIVKAII, GP3, CSTB, SCCA1 and HGF for the diagnosis of hepatocellular carcinoma in patients with alcoholic liver cirrhosis. [pubmed:28766361]
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