窖蛋白(CAV1)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Caveolin 1 (CAV1)

VIP21; Caveolae Protein 22kDa

  • 窖蛋白(CAV1)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 窖蛋白(CAV1)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 窖蛋白(CAV1)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA214Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测窖蛋白(CAV1)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将窖蛋白(CAV1)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的窖蛋白(CAV1)与连接于固相载体上的抗体结合，然后加入生物素化的窖蛋白(CAV1)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的窖蛋白(CAV1)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
RPA214Hu01 窖蛋白(CAV1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
CPA214Hu21 窖蛋白(CAV1)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA214Hu08 窖蛋白(CAV1)多克隆抗体 WB; IHC; ICC; IP.
PAA214Hu01 窖蛋白(CAV1)多克隆抗体 WB; IHC; FCM
MAA214Hu22 窖蛋白(CAV1)单克隆抗体 WB; IHC; ICC; IP.
SEA214Hu 窖蛋白(CAV1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA214Hu 窖蛋白(CAV1)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA214Hu01 窖蛋白(CAV1)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Mendel Net Novel prostate cancer tumour markers in a cell line model [Mendelu: source]
Oncology reports Caveolin-1 as a potential high-risk prostate cancer biomarker [PubMed: 22159333]
Anticancer Research Increased plasma caveolin-1 levels are associated with progression of prostate cancer among Japanese men [Pubmed: 23645736]
Sarcoidosis Vasc Diffuse Lung Dis.? Decrased sputum caveolin-1 is associated with systemic sclerosis related lung disease [Pubmed:24751454]
Cancer Genomics and Proteomics Significant Association of Caveolin-1 and Caveolin-2 with Prostate Cancer Progression [PubMed: 26543085]
Atherosclerosis Genomic variant in CAV1 increases susceptibility to coronary artery disease and myocardial infarction [Pubmed:26775120]
Arthritis Research & Therapy Increased caveolin-1 in intervertebral disc degeneration facilitates repair [Pubmed:26939667]
Oxid Med Cell Longev Lower Serum Caveolin-1 Is Associated with Cerebral Microbleeds in Patients with Acute Ischemic Stroke [Pubmed:27119011]
Inflammation Caveolin-1 Promotes the Imbalance of Th17/Treg in Patients with Chronic Obstructive PulmonaryDisease. [pubmed:27613621]
Oxidative Medicine and Cellular Longevity Swim Training Modulates Skeletal Muscle Energy Metabolism, Oxidative Stress, and Mitochondrial Cholesterol Content in Amyotrophic Lateral Sclerosis Mice [Pubmed:29849903]
Microcirculation Endothelium‐dependent hyperpolarization‐mediated vasodilatation compensates nitric oxide‐mediated endothelial dysfunction during ischemia in diabetes‐induced … [Pubmed:29665152]
Genetic Testing and Molecular Biomarkers The CAV1 Gene 3′ Untranslated Region Single Nucleotide Polymorphisms Are Associated with the Risk of Pulmonary Hypertension in Chinese Han Chronic … []
Cell Rep SIRT6 transcriptionally regulates fatty acid transport by suppressing PPAR¦Ã [34077730]
researchsquare Cav-1 Protein Levels in Serum and Infarcted Brain Correlate with Hemorrhagic Volume in a Mouse Model of Thromboembolic Stroke, Independently of Rt-PA?¡­ []
Molecules 5, 2′-Dibromo-2, 4′, 5′-trihydroxydiphenylmethanone Inhibits LPS-Induced Vascular Inflammation by Targeting the Cav1 Protein [Pubmed:35566232]
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