含Ⅲ型纤连蛋白域蛋白5(FNDC5)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Fibronectin Type III Domain Containing Protein 5 (FNDC5) ,etc. by FLIA (Flow Luminescence Immunoassay)

FRCP2; Irisin; Fibronectin type III repeat-containing protein 2

（注：单次混测多因子不超过8个指标 ）

  • 含Ⅲ型纤连蛋白域蛋白5(FNDC5)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 含Ⅲ型纤连蛋白域蛋白5(FNDC5)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测含Ⅲ型纤连蛋白域蛋白5(FNDC5)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的含Ⅲ型纤连蛋白域蛋白5(FNDC5)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 91-99 94
EDTA plasma(n=5) 81-98 93
heparin plasma(n=5) 98-105 101
sodium citrate plasma(n=5) 89-97 93

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的含Ⅲ型纤连蛋白域蛋白5(FNDC5)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中含Ⅲ型纤连蛋白域蛋白5(FNDC5)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 82-102% 80-91% 93-101% 96-103%
EDTA plasma(n=5) 84-97% 84-91% 91-104% 80-95%
heparin plasma(n=5) 80-89% 91-99% 92-105% 81-98%
sodium citrate plasma(n=5) 80-96% 90-98% 79-102% 96-105%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
    37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。

实验原理

将含Ⅲ型纤连蛋白域蛋白5(FNDC5)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的含Ⅲ型纤连蛋白域蛋白5(FNDC5)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的含Ⅲ型纤连蛋白域蛋白5(FNDC5)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的含Ⅲ型纤连蛋白域蛋白5(FNDC5)等多因子检测试剂盒(流式荧光发光法)呈正相关。

赠品

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
RPN576Hu05 含Ⅲ型纤连蛋白域蛋白5(FNDC5)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPN576Hu06 含Ⅲ型纤连蛋白域蛋白5(FNDC5)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPN576Hu07 含Ⅲ型纤连蛋白域蛋白5(FNDC5)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPN576Hu02 含Ⅲ型纤连蛋白域蛋白5(FNDC5)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPN576Hu61 含Ⅲ型纤连蛋白域蛋白5(FNDC5)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPN576Hu03 含Ⅲ型纤连蛋白域蛋白5(FNDC5)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPN576Hu01 含Ⅲ型纤连蛋白域蛋白5(FNDC5)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APN576Hu01 含Ⅲ型纤连蛋白域蛋白5(FNDC5)活性蛋白 Cell culture; Activity Assays.
APN576Hu61 含Ⅲ型纤连蛋白域蛋白5(FNDC5)活性蛋白 Cell culture; Activity Assays.
RPN576Hu04 含Ⅲ型纤连蛋白域蛋白5(FNDC5)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAN576Hu02 含Ⅲ型纤连蛋白域蛋白5(FNDC5)多克隆抗体 WB; IHC; ICC; IP.
PAN576Hu04 含Ⅲ型纤连蛋白域蛋白5(FNDC5)多克隆抗体 WB; IHC; ICC; IP.
PAN576Hu01 含Ⅲ型纤连蛋白域蛋白5(FNDC5)多克隆抗体 WB; IHC; ICC; IP.
LAN576Hu71 含Ⅲ型纤连蛋白域蛋白5(FNDC5)多克隆抗体(生物素标记) WB; IHC; ICC.
SEN576Hu 含Ⅲ型纤连蛋白域蛋白5(FNDC5)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMN576Hu 含Ⅲ型纤连蛋白域蛋白5(FNDC5)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Diabetes Research and Clinical Practice Serum irisin levels in new-onset type 2 diabetes [PubMed: 23369227]
Journal of diabetes and its complications Lower circulating irisin is associated with type 2 diabetes mellitus [Pubmed: 23619195]
Horm Metab Res. Inconsistency in circulating irisin levels: what is really happening [Pubmed:24459033]
Int J Obes (Lond). Leptin administration activates irisin-induced myogenesis via nitric oxide-dependent mechanisms, but reduces its effect on subcutaneous fat browning in mice [Pubmed:25199621]
Molecular and Cellular Endocrinology Fenofibrate (PPARalpha agonist) induces beige cell formation in subcutaneous white adipose tissue from diet-induced male obese mice [Pubmed:25576856]
journal of diabetes Lower irisin level in patients with type 2 diabetes mellitus: A case-control study and meta-analysis [PubMed: 25494632]
The Journal of Maternal-Fetal & Neonatal Medicine Maternal circulating levels of irisin in intrahepatic cholestasis of pregnancy [PubMed: 26689349]
Hormone and Metabolic Research Lower Circulating Irisin Level in Patients with Diabetes Mellitus: A Systematic Review and Meta-Analysis [Pubmed:27300472]
Nature Reviews Endocrinology Physiology and role of irisin in glucose homeostasis. [pubmed:28211512]
Journal of Molecular and Cellular Cardiology Irisin improves perivascular adipose tissue dysfunction via regulation of the heme oxygenase-1/adiponectin axis in diet-induced obese mice [pubmed:27638193]
Biochemical and Biophysical Research Communications Irisin stimulates cell proliferation and invasion by targeting the PI3K/AKT pathway in human hepatocellular carcinoma [pubmed:28867187]
PLoS One Differential actions of PPAR-α and PPAR-β/δ on beige adipocyte formation: A study in the subcutaneous white adipose tissue of obese male mice [Pubmed:29351550]
International Brazilian Journal of Urology The Diagnostic Value of FNDC5/Irisin in Renal Cell Cancer. [Pubmed:29522296]
Redox Biology Irisin alleviates liver ischemia-reperfusion injury by inhibiting excessive mitochondrial fission, promoting mitochondrial biogenesis and decreasing oxidative … [Doi: 10.1016/j.redox.2018.10.019]
Antioxidants & redox signaling Low serum irisin concentration is associated with poor outcomes in patients with acute pancreatitis and irisin administration protects against experimental acute … [Pubmed: 31250660]
Journal of Maternal-Fetal & Neonatal Medicine Maternal serum, placental, and umbilical venous blood irisin levels in intrahepatic cholestasis of pregnancy [Pubmed: 31590596]
Biomed Pharmacother Identification of irisin as a therapeutic agent that inhibits oxidative stress and fibrosis in a murine model of chronic pancreatitis [Pubmed: 32199226]
Irisin Improves Autophagy of Aged Hepatocytes via Increasing Telomerase Activity in Liver Injury [Pubmed: 31976032]
Serum Irisin Predicts Posthepatectomy Complications in Patients with Hepatocellular Carcinoma [Pubmed: 31976024]
DOMESTIC ANIMAL ENDOCRINOLOGY The myokine Irisin: localization and effects in swine late medium and large antral ovarian follicle [Pubmed: 33120167]
Journal of Biomaterials and Tissue Engineering The Combination of Aerobic and Resistance Exercise Induces Weight Loss via the PGC-1α/Irisin/UCP-1 Pathway []
Evaluation of a recombinant bacillus calmette-guérin vaccine expressing P39-L7/L12 of Brucella melitensis: An immunization strategy against brucellosis in BALB/c … []
Eur Rev Med Pharmacol Sci Salivary Irisin: potential inflammatory biomarker in recurrent apthous stomatitis patients [33755963]
Metabolites Pattern of Adiponectin, Osteocalcin, Irisin, FGF-21, and MCP-1 According to the Body Size Phenotype: Could They Be Markers of Metabolic Health in Mexican-Mestizo … [34822430]
Eat Weight Disord Time-restricted feeding prevents metabolic diseases through the regulation of galanin/GALR1 expression in the hypothalamus of mice [34370270]
Ir J Med Sci Eight-week exercise program improved the quality of life of Alzheimer's patients through functional, cognitive, and biochemical parameters [Pubmed:35604540]
Biomed Pharmacother Irisin attenuates sepsis-induced cardiac dysfunction by attenuating inflammation-induced pyroptosis through a mitochondrial ubiquitin ligase-dependent … [Pubmed:35653888]
Journal of King Saud University - Science Interaction of nitric oxide synthase and mitochondrial ATP-sensitive potassium channels in protective impacts of combination therapy with irisin … []
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