肌肉生长抑制素(MSTN)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Myostatin (MSTN) ,etc. by FLIA (Flow Luminescence Immunoassay)

GDF8; Growth Differentiation Factor 8

(注:单次混测多因子不超过8个指标 )

  • 肌肉生长抑制素(MSTN)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 肌肉生长抑制素(MSTN)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测肌肉生长抑制素(MSTN)等多因子检测试剂盒(流式荧光发光法),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的肌肉生长抑制素(MSTN)等多因子检测试剂盒(流式荧光发光法)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 96-103 99
EDTA plasma(n=5) 86-94 90
heparin plasma(n=5) 90-104 97
sodium citrate plasma(n=5) 83-97 93

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的肌肉生长抑制素(MSTN)等多因子检测试剂盒(流式荧光发光法),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中肌肉生长抑制素(MSTN)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 78-102% 81-105% 94-102% 89-101%
EDTA plasma(n=5) 83-102% 96-105% 95-102% 98-105%
heparin plasma(n=5) 84-97% 94-102% 95-102% 80-88%
sodium citrate plasma(n=5) 97-105% 89-103% 98-105% 96-105%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备;
2. 加样(标准品、样本、磁珠、检测溶液A)标准品或样本50μL及磁珠10μL,加检测溶液A50μL,
    37°C酶标板振荡器孵育60分钟;
3. 磁吸洗板3次;
4. 加检测溶液B100μL,37°C振动孵育30分钟;
5. 磁吸洗板3次;
6. 加鞘液100μL,旋涡震荡2分钟后读数。

实验原理

将肌肉生长抑制素(MSTN)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠,制成固相载体,向微孔中分别加入标准品或标本以及磁珠、标记VEGFA,标记的肌肉生长抑制素(MSTN)等多因子检测试剂盒(流式荧光发光法)和未标记的肌肉生长抑制素(MSTN)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合,然后将未结合物洗净后,加入PE标记的亲和素,再次彻底洗涤后即可上机读数。MFI值和样品中的肌肉生长抑制素(MSTN)等多因子检测试剂盒(流式荧光发光法)呈负相关。

赠品

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPB653Mu02 肌肉生长抑制素(MSTN)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB653Mu01 肌肉生长抑制素(MSTN)活性蛋白 Cell culture; Activity Assays.
RPB653Mu01 肌肉生长抑制素(MSTN)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
CPB653Mu21 肌肉生长抑制素(MSTN)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAB653Mu08 肌肉生长抑制素(MSTN)多克隆抗体 WB; IHC; ICC; IP.
PAB653Mu02 肌肉生长抑制素(MSTN)多克隆抗体 WB; IHC; ICC; IP.
PAB653Mu01 肌肉生长抑制素(MSTN)多克隆抗体 WB; IHC; ICC; IP.
LAB653Mu71 肌肉生长抑制素(MSTN)多克隆抗体(生物素标记) WB; IHC; ICC.
SEB653Mu 肌肉生长抑制素(MSTN)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
CEB653Mu 肌肉生长抑制素(MSTN)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB653Mu 肌肉生长抑制素(MSTN)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB653Mu11 肌肉生长抑制素(MSTN)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
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Calcif Tissue Int. The Effect of Vibration Treatments Combined with Teriparatide or Strontium Ranelate on Bone Healing and Muscle in Ovariectomized Rats [Pubmed:27272029]
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Calcified Tissue International Effect of Strontium Ranelate on the Muscle and Vertebrae of Ovariectomized Rats [pubmed:29242963]
Scientific Reports Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis. [pubmed:28740214]
Journal of Animal Science Fermented corn-soybean meal elevated IGF1 levels in grower-finisher pigs [Pubmed: 30203098]
European Journal of Internal Medicine Predictive value of serum myostatin for the severity and clinical outcome of heart failure [Pubmed: 31056368]
J Cell Physiol Resistance training‐induced muscle hypertrophy is mediated by TGF‐β1‐Smad signaling pathway in male Wistar rats [Pubmed: 31960436]
NEPHROLOGY DIALYSIS TRANSPLANTATION Myostatin and muscle atrophy during chronic kidney disease [Pubmed: 32974666]
Oxid Med Cell Longev Gender-Specific Behaviour in Obesity Stages I-II: Imbalance of Aminothiol Status and Adipomyokine Profile in Subjects with Different Insulin Resistance … [34868459]
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