血管活性肠肽(VIP)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Vasoactive Intestinal Peptide (VIP) ,etc. by FLIA (Flow Luminescence Immunoassay)
PHM27; Intestinal peptide PHV-42; Peptide histidine valine 42; Peptide histidine methioninamide 27
（注：单次混测多因子不超过8个指标 ）
- 编号LMA380Ra
- 物种Rattus norvegicus (Rat，大鼠) 相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长1.5h
- 检测范围0.49-500pg/mL
- 灵敏度最小可检测剂量小于等于0.163 pg/mL.
- 样本类型Serum, plasma and other biological fluids
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产品包装(模拟)
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产品包装(模拟)
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测血管活性肠肽(VIP)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的血管活性肠肽(VIP)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 80-91 | 86 |
| EDTA plasma(n=5) | 92-104 | 101 |
| heparin plasma(n=5) | 88-104 | 97 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的血管活性肠肽(VIP)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中血管活性肠肽(VIP)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 82-91% | 98-105% | 92-99% | 87-101% |
| EDTA plasma(n=5) | 92-103% | 87-95% | 95-105% | 78-95% |
| heparin plasma(n=5) | 91-102% | 78-92% | 78-98% | 82-104% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠、检测溶液A）标准品或样本50μL及磁珠10μL，加检测溶液A50μL，
37°C酶标板振荡器孵育60分钟；
3. 磁吸洗板3次；
4. 加检测溶液B100μL，37°C振动孵育30分钟；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将血管活性肠肽(VIP)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠、标记VEGFA，标记的血管活性肠肽(VIP)等多因子检测试剂盒(流式荧光发光法)和未标记的血管活性肠肽(VIP)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合，然后将未结合物洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的血管活性肠肽(VIP)等多因子检测试剂盒(流式荧光发光法)呈负相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA380Ra01 | 血管活性肠肽(VIP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA380Ra01 | 血管活性肠肽(VIP)多克隆抗体 | WB; IHC; ICC; IP. |
| MAA380Ra21 | 血管活性肠肽(VIP)单克隆抗体 | WB; IHC; ICC; IP. |
| CEA380Ra | 血管活性肠肽(VIP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA380Ra | 血管活性肠肽(VIP)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA380Ra11 | 血管活性肠肽(VIP)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Clinical Genetics | VIP gene variants related to idiopathic pulmonary arterial hypertension in Chinese population [Wiley: source] |
| Human immunology | Low levels of vasoactive intestinal peptide are associated with Chagas disease cardiomyopathy [Pubmed: 23800435] |
| Headache: The Journal of Head and Face Pain | CGRP and VIP Levels as Predictors of Efficacy of Onabotulinumtoxin Type A in Chronic Migraine [Onlinelibrary: head.12372] |
| World J Gastroenterol | Roles of sphincter of Oddi motility and serum vasoactive intestinal peptide, gastrin and cholecystokinin octapeptide [NCBI: PMC4000510] |
| Cephalalgia? | Increased VIP levels in peripheral blood outside migraine attacks as a potential biomarker of cranial parasympathetic activation in chronic migraine [Pubmed:24847167] |
| J Huazhong Univ Sci Technolog Med Sci. | Clinical study of electro-acupuncture treatment with different intensities for functional constipation patients [Pubmed:25318892] |
| PLOS ONE | Aqueous Fraction of Beta vulgaris Ameliorates Hyperglycemia in Diabetic Mice due to Enhanced Glucose Stimulated Insulin Secretion, Mediated by Acetylcholine and GLP-1, and Elevated Glucose Uptake via Increased Membrane Bound GLUT4 Transporters [Pubmed:25647228] |
| PLOS ONE | Aqueous Fraction of [PubMed: 25647228] |
| Pharmacol Res | Oral curcumin has anti-arthritic efficacy through somatostatin generation [PubMed: 25836921] |
| World J Gastroenterol | Effects of sphincter of Oddi motility on the formation of cholesterol gallstones [Pubmed:27350732] |
| Annals of Allergy, Asthma and Immunology | Effect of acupuncture on house dust mite specific IgE, substance P, and symptoms in persistent allergic rhinitis [Pubmed:27156748] |
| Cephalalgia. | Relationship between serum levels of VIP, but not of CGRP, and cranial autonomic parasympathetic symptoms: A study in chronic migraine patients [Pubmed:27250233] |
| Headache | No Change in Interictal PACAP Levels in Peripheral Blood in Women With Chronic Migraine [pubmed:27634731] |
| Sci Rep. | Using the "target constituent removal combined with bioactivity assay" strategy to investigate the optimum arecoline content in charred areca nut. [pubmed:28054652] |
| BMC Complementary and Alternative Medicine | [6]-Gingerol, from Zingiber officinale, potentiates GLP-1 mediated glucose-stimulated insulin secretion pathway in pancreatic β-cells and increases RAB8/RAB10-regulated membrane presentation of GLUT4 transporters in skeletal muscle to improve hyperglycemia in Leprdb/db type 2 diabetic mice [10.1186/s12906-017-1903-0] |
| Applied Biological Chemistry | Positive enhancement of Lactobacillus fermentum HY01 on intestinal movements of mice having constipation [10.1007/s13765-017-0327-3] |
| Journal of Periodontology | Salivary neuropeptides, stress, and periodontitis [Pubmed:28914594] |
| Evidence-Based Complementary and Alternative Medicine | Home-Based Transcutaneous Neuromodulation Improved Constipation via Modulating Gastrointestinal Hormones and Bile Acids [Pubmed:29853946] |
| Revista do Instituto de Medicina Tropical de São Paulo | Vasoactive intestinal peptide degradation might influence Interleukin-17 expression in cardiac chagasic patients [Pubmed: 30365640] |
| Journal of Veterinary Science | Serum Levels of Neuropeptides in Cows with Left Abomasal Displacement [Pubmed: 30562932] |
| Science of Food and Agriculture | Protective Effect of Silkworm Pupa Oil on Hydrochloric Acid/Ethanol‐Induced Gastric Ulcer [Pubmed: 30479041] |
| MOLECULAR BIOLOGY REPORTS | Serum CGRP, VIP, and PACAP usefulness in migraine: a case–control study in chronic migraine patients in real clinical practice [Pubmed: 32951099] |
| Journal of Cystic Fibrosis | Disrupted local innervation results in less VIP expression in CF mice tissues [Pubmed: 32600901] |
| Biomedical Journal of Scientific & Technical Research | Anti-Inflammatory and Pro-Inflammatory Neuropeptides in Dilative Cardiomyopathy are there Relations to Psychophysical Stress? [] |
| Journal of tranditional chinese medical sciences | Ganjiang granule regulates cecal microflora and serum biochemical components in a rat model of constipation-predominant irritable bowel syndrome [] |
| J Cyst Fibros | VIP reduction in the pancreas of F508del homozygous CF mice and early signs of Cystic Fibrosis Related Diabetes (CFRD) [34034984] |
| Neurol Sci | Significance of serum VIP and PACAP in multiple sclerosis: an exploratory case–control study [34698942] |
| Endocr J | A clinical analysis on functioning pancreatic neuroendocrine tumors (focusing on VIPomas): a single-center experience [Pubmed:35644576] |
